Methods in Molecular Biology 2010-01-01

Metabolic engineering of glyoxalase pathway for enhancing stress tolerance in plants.

Ananda Mustafiz, Khirod K Sahoo, Sneh L Singla-Pareek, Sudhir K Sopory

Index: Methods Mol. Biol. 639 , 95-118, (2010)

Full Text: HTML

Abstract

Glyoxalase system consists of two enzymes glyoxalase I (Gly I) and glyoxalase II (Gly II). Gly I detoxifies methylglyoxal (MG), a cytotoxic byproduct of glycolysis, to S-lactoylglutathione (SLG) where it uses one molecule of reduced glutathione. Subsequently, SLG is converted to lactate by Gly II and one molecule of reduced glutathione is recycled back into the system. The level of MG, which is produced ubiquitously in all living organisms, is enhanced upon exposure to different abiotic stresses in plants. Overexpression of glyoxalase pathway genes in transgenic plants has been found to keep a check on the MG level under stress conditions, regulate glutathione homeostasis, and the transgenic plants are able to survive and grow under various abiotic stresses.