Journal of Analytical Toxicology 1989-01-01

Specificity data of the salicylate assay by fluorescent polarization immunoassay.

M Koel, P Nebinger

Index: J. Anal. Toxicol. 13(6) , 358-60, (1989)

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Abstract

We studied the recently developed Abbott Laboratories fluorescent polarization immunoassay (FPIA) for salicylates in serum. The cross-reactivity test of the assay was performed with 20 substances that showed a similar chemical structure as salicylic acid. A chemical substitution on the 5- position of the salicylate formula enhanced the cross-reactivity. 5-Methylsalicylic acid, diflunisal, salazosulfapyridin, and 5-aminosalicylic acid (5-ASA) showed a significant cross-reactivity with 1200%, 222%, 153% and 122%, respectively. Diflunisal and salazosulfapyridin at a similar therapeutic serum level as salicylic acid can produce false positive salicylate results, in case of unknown medication of these compounds.