FEBS Letters 1987-02-09

NMR study of the interaction of P-450 with 4-methoxypyridine.

Woldman YaYu, L M Weiner, L F Gulayeva, V V Lyakhovich

Index: FEBS Lett. 212(1) , 53-7, (1987)

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Abstract

Longitudinal relaxation (T1) measurements for all lines (alpha-CH, beta-CH, O-CH3) in the 4-methoxypyridine 1H-NMR spectrum were used to study the interaction of 4-methoxypyridine with purified microsomal cytochrome P-450 from livers of phenobarbital-treated rats. The paramagnetic contribution to the observed T1(-1) value was determined from its dependence on 4-methoxypyridine concentration. In the P-450-4-methoxypyridine complex the latter is oriented so that the nitrogen of pyridine is directed towards the Fe3+.