Michael H Nantz, Christopher W Dicus, Brendan Hilliard, Sri Yellayi, Shaomin Zou, James G Hecker
Index: Mol. Pharm. 7(3) , 786-94, (2010)
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We, and others, have observed that the structure of cationic lipids appears to have a significant effect on the transfection efficacy of optimized nucleic acid/cationic lipid complexes (lipoplexes) used for in vitro and in vivo gene delivery and expression. Although there are many in vitro comparisons of lipid reagents for gene delivery, few comparisons have been made in vivo. We previously reported the effects of changes in hydrophobic domain chain length and chain asymmetry, changes in headgroup composition, and counterion exchange. We have observed in our own work over many years the apparent superiority of asymmetric versus symmetric hydrocarbon domains for otherwise similar lipids. In this investigation we use in vivo whole animal brain imaging to evaluate the contribution of symmetric versus asymmetric hydrophobic domains on what we previously determined to be optimal chain lengths for in vitro transfections. We specifically investigated several glycerol-based lipids; however, the rare reports of asymmetric non-glycerol-based lipids also support our observations. We found that asymmetric, two-chain cationic lipids of 14 to 18 carbons perform significantly better in vivo, as analyzed by whole animal imaging, than the paired symmetric lipids.
Structure | Name/CAS No. | Molecular Formula | Articles |
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Myristic Anhydride
CAS:626-29-9 |
C28H54O3 |
Inhibition of N-Methyl-D-aspartate-induced Retinal Neuronal ...
2015-09-04 [J. Biol. Chem. 290(36) , 22030-48, (2015)] |
A convenient synthesis of phosphatidylcholines: acylation of...
1979-07-01 [J. Lipid Res. 20(5) , 674-7, (1979)] |
Synthesis of saturated, unsaturated, spin-labeled, and fluor...
[Lipids 14(9) , 816-818, (1979)] |
Reactions of fatty acid chlorides. I. Preparation of fatty a...
[J. Am. Oil Chem. Soc. 31(4) , 151-157, (1954)] |
Neryl myristate from the acarid mite, Aleuroglyphus ovatu...
[Agric. Biol. Chem. 52(5) , 1299-1300, (1988)] |
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