Bioscience, Biotechnology, and Biochemistry 1996-05-01

Bestatin analogue from Streptomyces neyagawaensis SL-387.

M C Chung, H J Lee, H K Chun, C H Lee, S I Kim, Y H Kho

Index: Biosci. Biotechnol. Biochem. 60 , 898-900, (1996)

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Abstract

A bestatin analogue, (2S,3R)-3-amino-2-hydroxy-4-phenylbutanoyl-L-valine (AHPA-Val), from the culture filtrate of Streptomyces neyagawaensis SL-387 was obtained in a chemically defined medium containing DL-3-amino-3-phenylpropionic acid. AHPA-Val was 6 times (IC50 = 1.2 micrograms/ml) as strong as bestatin (IC50 = 7.0 micrograms/ml) against porcine kidney microsomal aminopeptidase N, and 4 times (5.6 micrograms/ml) as strong as bestatin (IC50 = 20.7 micrograms/ml) against aminopeptidase N of human metastatic fibrosarcoma HT1080. To the best of our knowledge, this is the first report on the microbial production of AHPA-Val.