Bioprocess and Biosystems Engineering 2011-02-01

Immobilization of Thermoanaerobium brockii alcohol dehydrogenase on SBA-15.

Michela Vittorini, Emil Dumitriu, Gabriel Barletta, Francesco Secundo

Index: Bioprocess Biosyst. Eng. 34(2) , 247-51, (2011)

Full Text: HTML

Abstract

The activity of Thermoanaerobium brockii alcohol dehydrogenase (TBADH) adsorbed on mesoporous silica SBA-15 was compared with that of the free enzyme in water and in biphasic system (water phase up to 50% v/v water). TBADH was active at a water concentration ≥10% v/v. In the reduction reaction of sulcatone to sulcatol carried out in biphasic systems, the yield obtained with SBA-15-adsorbed TBADH was up to 5.5-fold higher than that with the free enzyme, which suggests a higher stability of the immobilized enzyme toward the organic solvent. The nature of the organic solvent substantially influenced the degree of conversion that, for example, was 7.4% in toluene and 31.6% in petroleum ether.

Related Compounds

Structure Name/CAS No. Articles
5-Hepten-2-ol,6-methyl- Structure 5-Hepten-2-ol,6-methyl-
CAS:1569-60-4