Guojie Liu, Junting Liu, Kenji Hara, Yanfang Wang, Yanping Yu, Lina Gao, Ling Li
Index: J. Chromatogr. B. Analyt. Technol. Biomed. Life Sci. 877(27) , 3054-8, (2009)
Full Text: HTML
Cyanide (CN) is a powerful poison and rapidly toxic agent. Because of its wide availability and high toxicity, quantification of CN in blood and urine is frequently required in clinical and forensic practice. We present a sensitive and less time consuming method based on solid-supported liquid–liquid extraction (SLE) and gas chromatography–mass spectrometry (GC–MS) with two-step derivatization for determination of CN in plasma and urine. Buffer solution, 1,3,5-tribromobenzene (internal standard) and benzaidehyde were added to sample to complete the first-step derivatization. Then the analytes were poured onto the column of diatomaceous earth, eluted with n-hexane containing 0.4% of heptafluorobutyryl chloride (HFB-Cl) to complete the second-step derivatization forming the final analyte, heptafluoro-butyric acid-alpha-cyanobenzyl ester. This method was linear ( r 2 = 0.9988, 0.9993), reproducible (intra-day RSD = 4.37–7.24%, 3.19–5.74%; inter-day RSD = 5.13–7.63%, 4.31–6.69%), accurate (recoveries = 90.58–115.56%, 93.01–114.6%) and sensitive (LOD = 0.04, 0.01 μg/mL) for plasma and urine, respectively. The total time was about 25 min. This method was successfully applied to the analysis of blood sample and urine sample collected from a victim who died as a result of ingestion of potassium cyanide.
Structure | Name/CAS No. | Molecular Formula | Articles |
---|---|---|---|
![]() |
1,3,5-Tribromobenzene
CAS:626-39-1 |
C6H3Br3 |
Co-extraction and simultaneous determination of multi-class ...
2015-10-01 [Talanta 143 , 7-18, (2015)] |
PBDEs and other POPs in urban birds of prey partly explained...
2015-08-15 [Sci. Total Environ. 524-525 , 157-65, (2015)] |
[Solid phase microextraction and derivatization of sulfide i...
2003-11-01 [Wei Sheng Yan Jiu 32(6) , 620-1, (2003)] |
Hepatotoxicity of brominated benzenes: relationship between ...
1998-01-01 [Arch. Toxicol. 72(2) , 97-103, (1998)] |
A novel 1,3,5-tris(alpha,beta,beta-trifluorovinyl)benzene mo...
2003-10-21 [Chem. Commun. (Camb.) (20) , 2596-7, (2003)] |
Home | MSDS/SDS Database Search | Journals | Product Classification | Biologically Active Compounds | Selling Leads | About Us | Disclaimer
Copyright © 2024 ChemSrc All Rights Reserved