Bioorganic Chemistry 2008-12-01

The putative coenzyme B12-dependent methylmalonyl-CoA mutase from potatoes is a phosphatase.

Csaba Paizs, Tanja Diemer, János Rétey

Index: Bioorg. Chem. 36 , 261-264, (2008)

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Abstract

The reported presence of a coenzyme B12-dependent methylmalonyl-CoA mutase in potatoes has been reexamined. The enzyme converting methylmalonyl-CoA was purified to electrophoretic homogeneity. Examination of the reaction product by 1H, 31P NMR and mass spectrometry revealed that it was methylmalonyl-3'-dephospho-CoA. The phosphatase enzyme needs neither coenzyme B12 nor S-adenosylmethionine as a cofactor.