Dansylcadaverine

Names

[ CAS No. ]:
10121-91-2

[ Name ]:
Dansylcadaverine

[Synonym ]:
Dansylcadaverine
N-(5-aminopentyl)-5-(dimethylamino)naphthalene-1-sulfonamide

Biological Activity

[Description]:

Dansylcadaverine is a fluorescent probe.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Dye Reagents

Research Areas >> Others

[In Vitro]

Detection of Dansylcadaverine fluorescence after SDS-PAGE shows that the amount of actin-thymosin β4 crosslink is very low in the presence of Dansylcadaverine and transglutaminase, while there are large amounts of Dansylcadaverine-labeled thymosin β4 and actin. The amount of cross-link further decreases in the presence of Dansylcadaverine competing for Lys residues of either G-actin or thymosin β4 (100 μM). In the absence of thymosin β4, Dansylcadaverine-labeled actin polymerized to F-actin as revealed by fluorescence detection after native PAGE[1].

[Kinase Assay]

To determine the time course of transglutamination, thymosin β4 (120 μM) is incubated with Dansylcadaverine (5 mM) in 70 μL buffer consisting of 10 mM Tris-HCl, pH 7.4, 15 mM CaCl2, 3 mM DTT. The reaction is started by addition of 0.1 U transglutaminase. Immediately after addition of the enzyme (t=0) and at indicated times,10 μL are taken from the mixture, diluted in 490 μL 0.1% TFA to stop the reaction and analyzed by HPLC[1].

[Cell Assay]

MCF7 cells (2.4×104) are seeded into 35 mm plates. After 24 h incubation, CuO NPs are added with an increasing concentration in the presence or absence of 3-Methyladenine (3-MA) for different time periods . The cells are then incubated with 50 mM Dansylcadaverine (MDC) at 37°C for 15 min and washed with 1×PBS three times with 5 min interval. Finally, the cells are observed under a fluorescence microscope[2].

[References]

[1]. Huff T, et al. Thymosin beta(4) serves as a glutaminyl substrate of transglutaminase. Labeling with fluorescentdansylcadaverine does not abolish interaction with G-actin. FEBS Lett. 1999 Dec 24;464(1-2):14-20.

[2]. Laha D, et al. Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7. Biochim Biophys Acta. 2014 Jan;1840(1):1-9.

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.19g/cm3

[ Boiling Point ]:
505.5ºC at 760mmHg

[ Melting Point ]:
137-140ºC

[ Molecular Formula ]:
C₁₇H₂₅N₃O₂S

[ Molecular Weight ]:
335.46400

[ Flash Point ]:
259.5ºC

[ Exact Mass ]:
335.16700

[ PSA ]:
83.81000

[ LogP ]:
4.48510

[ Vapour Pressure ]:
9.54E-11mmHg at 25°C

[ Index of Refraction ]:
1.594

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
26-36

[ RIDADR ]:
NONH for all modes of transport

[ HS Code ]:
2935009090

Customs

[ HS Code ]: 2935009090

[ Summary ]:
2935009090 other sulphonamides VAT:17.0% Tax rebate rate:9.0% Supervision conditions:none MFN tariff:6.5% General tariff:35.0%

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Related Compounds

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