5-(((2,5-DIOXOPYRROLIDIN-1-YL)OXY)CARBONYL)-3-OXO-3H-SPIRO[ISOBENZOFURAN-1,9'-XANTHENE]-3',6'-DIYL DIACETATE

Names

[ Name ]:
5-(((2,5-DIOXOPYRROLIDIN-1-YL)OXY)CARBONYL)-3-OXO-3H-SPIRO[ISOBENZOFURAN-1,9'-XANTHENE]-3',6'-DIYL DIACETATE

[Synonym ]:
Carboxyfluorescein diacetate succinimidyl ester
2,5-Pyrrolidinedione, 1-[[[3',6'-bis(acetyloxy)-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthen]-5-yl]carbonyl]oxy]-
5-Carboxyfluorescein diacetate N-Hydroxysuccinimide Ester
5-{[(2,5-Dioxo-1-pyrrolidinyl)oxy]carbonyl}-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-3',6'-diyl diacetate
5-CFDA N-succinimidyl ester
(2,5-dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylate
5-Carboxy-di-O-acetylfluorescein N-succinimidyl ester

Biological Activity

[Description]:

5-Carboxyfluorescein diacetate N-succinimidyl ester is a cell permeable dye (Ex=492 nm, Em=517 nm). 5-Carboxyfluorescein diacetate N-succinimidyl ester can label cells by covalently binding to intracellular molecules. 5-Carboxyfluorescein diacetate N-succinimidyl ester is used to track lymphocyte migration and proliferation[1].

[Related Catalog]:

Research Areas >> Others

Signaling Pathways >> Others >> Others

[In Vitro]

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1]. 1. Dilute the stock solution in PBS (to give a 50 μM solution). 2. Add 110 μL of this solution per milliliter of cells (to give a final concentration of 5 μM) and mix rapidly. 3. After 5 min at room temperature, add 10 vol of PBS containing 5% FBS. 4. Centrifuge cells 5 min at 300 ×g, 20 ℃. 5. Remove the supernatant, and wash threetimes, each time by resuspending in 10 vol PBS containing 5% FBS. 6. Centrifuge cells 5 min at 300 × g, 20 ℃, and removing the supernatant. Note: Labeling occurs rapidly, and it is essential that the tracker is dispersed as evenly and quickly as possible so that cells are uniformly labeled.

[References]

[1]. Christopher R Parish, et al. Use of the intracellular fluorescent dye CFSE to monitor lymphocyte migration and proliferation. Curr Protoc Immunol. 2009 Feb;Chapter 4:Unit4.9.

Chemical & Physical Properties

[ Density]:
1.6±0.1 g/cm3

[ Boiling Point ]:
757.9±70.0 °C at 760 mmHg

[ Melting Point ]:
152-154ºC(lit.)

[ Molecular Formula ]:
C₂₉H₁₉NO₁₁

[ Molecular Weight ]:
557.461

[ Flash Point ]:
412.2±35.7 °C

[ Exact Mass ]:
557.095825

[ PSA ]:
151.81000

[ LogP ]:
0.50

[ Vapour Pressure ]:
0.0±2.6 mmHg at 25°C

[ Index of Refraction ]:
1.701

MSDS

Click to get detail MSDS

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter

[ Hazard Codes ]:
T

[ Risk Phrases ]:
R45

[ Safety Phrases ]:
S53

[ RIDADR ]:
NONH for all modes of transport

Articles

MHC class II presentation is controlled by the lysosomal small GTPase, Arl8b.

J. Immunol. 194(5) , 2079-88, (2015)

Dendritic cells (DCs) are specialized APCs with the ability to prime naive T cells. DCs first sample Ags from the environment and then orchestrate their processing and loading onto MHC class II (MHC I...

Coculture of peripheral blood-derived mesenchymal stem cells and endothelial progenitor cells on strontium-doped calcium polyphosphate scaffolds to generate vascularized engineered bone.

Tissue Eng. Part A 21(5-6) , 948-59, (2015)

Vascularization of engineered bone tissue is critical for ensuring its survival after implantation and it is the primary factor limiting its clinical use. A promising approach is to prevascularize bon...

Olanzapine inhibits proliferation, migration and anchorage-independent growth in human glioblastoma cell lines and enhances temozolomide's antiproliferative effect.

J. Neurooncol. 122(1) , 21-33, (2015)

The poor prognosis of patients with glioblastoma fuels the search for more effective therapeutic compounds. We previously hypothesised that the neuroleptic olanzapine may enhance antineoplastic effect...

Related Compounds

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