Evans Blue

Names

[ CAS No. ]:
314-13-6

[ Name ]:
Evans Blue

[Synonym ]:
Tetrasodium 6,6'-[(3,3'-dimethyl-4,4'-biphenyldiyl)di(E)-2,1-diazenediyl]bis(4-amino-5-oxido-3-sulfo-1-naphthalenesulfonate)
1,3-Naphthalenedisulfonic acid, 6,6'-[(3,3'-dimethyl[1,1'-biphenyl]-4,4'-diyl)di(E)-2,1-diazenediyl]bis[4-amino-5-hydroxy-, sodium salt (1:4)
evablin
EINECS 206-242-5
evans blue tetrasodium salt
6,6'-[(3,3'-Dimethyl[1,1'-biphenyl]-4,4'-diyl)bis(azo)]bis[4-amino-5-hydroxy-1,3-naphthalenedisulfonic Acid] Tetrasodium Salt
C.I. Direct Blue 53
Diazol Pure Blue BF
azovanblue
MFCD00004021
AZOVAN BLUE
GEIGY BLUE
t1824
4,4'-Bis[7-(1-amino-8-hydroxy-2,4-disulfo)naphthylazo]-3,3'-bitolyl Tetrasodium Salt
diazobleu
CI Direct Blue 53
blue Evan
Evans Blue
EVAN'S BLUE
Tetrasodium 6,6'-[(3,3'-dimethyl-4,4'-biphenyldiyl)di(E)-2,1-diazenediyl]bis(4-amino-5-hydroxy-1,3-naphthalenedisulfonate)
C.I. direct blue 53 tetrasodium salt
AZOVAN SODIUM
DiazoBlue
Direct Blue 53
Tetrasodium 6,6'-[(3,3'-dimethylbiphenyl-4,4'-diyl)di(E)diazene-2,1-diyl]bis(4-amino-5-hydroxynaphthalene-1,3-disulfonate)

Biological Activity

[Description]:

Evans Blue is a potent inhibitor of L-glutamate uptake via the membrane bound excitatory amino acid transporter (EAAT). Able to antagonize AMPA and kainate receptor mediated currents (IC50 values are 220 and 150 nM, respectively). IC50 value: 220/150 nMTarget: L-glutamate uptake receptorEvans blue is a biological dye, for detection of cell survival. Evans Blue can not penetrate healthy cell membrane, but can penetrate into death cell. Binds with high affinity to serum albumin.

[Related Catalog]:

Signaling Pathways >> Others >> Others

Biochemical Assay Reagents

Research Areas >> Others

[Related Small Molecules]

Chemical & Physical Properties

[ Molecular Formula ]:
C₃₄H₂₄N₆Na₄O₁₄S₄

[ Molecular Weight ]:
960.805

[ Exact Mass ]:
959.982422

[ PSA ]:
404.26000

[ LogP ]:
10.78520

[ Stability ]:
Stable. Incompatible with strong reducing agents, strong oxidizing agents.

[ Water Solubility ]:
280 G/L (20 ºC)

MSDS

Click to get detail MSDS

Toxicological Information

CHEMICAL IDENTIFICATION

RTECS NUMBER :: QJ6440000

CHEMICAL NAME :: 1,3-Naphthalenedisulfonic acid, 6,6'-((3,3'-dimethyl-4,4'-biphenylylene)bis(azo))bis (4-amino-5-hydroxy-, tetrasodium salt

CAS REGISTRY NUMBER :: 314-13-6

LAST UPDATED :: 199710

DATA ITEMS CITED :: 26

MOLECULAR FORMULA :: C34-H24-N6-O14-S4.4Na

MOLECULAR WEIGHT :: 960.84

WISWESSER LINE NOTATION :: L66J BZ CSWO ESWO JQ INUNR B1 DR C1 DNUN- IL66J BZ CSWO ESWO JQ &-NA- 4

HEALTH HAZARD DATA

ACUTE TOXICITY DATA

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 5 mL/kg

TOXIC EFFECTS :: Cardiac - other changes Lungs, Thorax, or Respiration - acute pulmonary edema

TYPE OF TEST :: LD50 - Lethal dose, 50 percent kill

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 340 mg/kg

TOXIC EFFECTS :: Details of toxic effects not reported other than lethal dose value

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Mammal - dog

DOSE/DURATION :: 3 mL/kg

TOXIC EFFECTS :: Gastrointestinal - hypermotility, diarrhea Gastrointestinal - nausea or vomiting Lungs, Thorax, or Respiration - acute pulmonary edema

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Mammal - cat

DOSE/DURATION :: 1 mL/kg

TOXIC EFFECTS :: Gastrointestinal - hypermotility, diarrhea Liver - other changes Kidney, Ureter, Bladder - changes in tubules (including acute renal failure, acute tubular necrosis)

TYPE OF TEST :: LDLo - Lowest published lethal dose

ROUTE OF EXPOSURE :: Intravenous

SPECIES OBSERVED :: Rodent - rabbit

DOSE/DURATION :: 1 mL/kg

TOXIC EFFECTS :: Cardiac - other changes Lungs, Thorax, or Respiration - acute pulmonary edema

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - mouse

DOSE/DURATION :: 620 mg/kg/10D-I

TOXIC EFFECTS :: Related to Chronic Data - death

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

SPECIES OBSERVED :: Rodent - rat

DOSE/DURATION :: 850 mg/kg/34W-I

TOXIC EFFECTS :: Tumorigenic - equivocal tumorigenic agent by RTECS criteria Liver - tumors Blood - leukemia

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 70 mg/kg

SEX/DURATION :: female 8 day(s) after conception

TOXIC EFFECTS :: Reproductive - Fertility - post-implantation mortality (e.g. dead and/or resorbed implants per total number of implants) Reproductive - Specific Developmental Abnormalities - Central Nervous System Reproductive - Specific Developmental Abnormalities - eye/ear

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 70 mg/kg

SEX/DURATION :: female 8 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Embryo or Fetus - fetotoxicity (except death, e.g., stunted fetus)

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Intraperitoneal

DOSE :: 140 mg/kg

SEX/DURATION :: female 8 day(s) after conception

TOXIC EFFECTS :: Reproductive - Effects on Embryo or Fetus - other effects to embryo

TYPE OF TEST :: TDLo - Lowest published toxic dose

ROUTE OF EXPOSURE :: Subcutaneous

DOSE :: 150 mg/kg

SEX/DURATION :: female 7-9 day(s) after conception

TOXIC EFFECTS :: Reproductive - Specific Developmental Abnormalities - Central Nervous System

TYPE OF TEST :: Unscheduled DNA synthesis

TYPE OF TEST :: DNA adduct

MUTATION DATA

TYPE OF TEST :: Unscheduled DNA synthesis

TEST SYSTEM :: Rodent - hamster Liver

DOSE/DURATION :: 10 umol/L

REFERENCE :: MUREAV Mutation Research. (Elsevier Science Pub. B.V., POB 211, 1000 AE Amsterdam, Netherlands) V.1- 1964- Volume(issue)/page/year: 136,255,1984 *** REVIEWS *** IARC Cancer Review:Animal Limited Evidence IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972- Volume(issue)/page/year: 8,151,1975 IARC Cancer Review:Human No Adequate Data IMEMDT IARC Monographs on the Evaluation of Carcinogenic Risk of Chemicals to Man. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) V.1- 1972- Volume(issue)/page/year: 8,151,1975 IARC Cancer Review:Group 3 IMSUDL IARC Monographs, Supplement. (WHO Publications Centre USA, 49 Sheridan Ave., Albany, NY 12210) No.1- 1979- Volume(issue)/page/year: 7,56,1987 TOXICOLOGY REVIEW ADTEAS Advances in Teratology. (New York, NY) V.1-5, 1966-72. Discontinued. Volume(issue)/page/year: 1,131,1966 *** NIOSH STANDARDS DEVELOPMENT AND SURVEILLANCE DATA *** NIOSH OCCUPATIONAL EXPOSURE SURVEY DATA : NOES - National Occupational Exposure Survey (1983) NOES Hazard Code - T0844 No. of Facilities: 162 (estimated) No. of Industries: 3 No. of Occupations: 6 No. of Employees: 5350 (estimated) No. of Female Employees: 2829 (estimated)

Safety Information

[ Symbol ]:

GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H350

[ Precautionary Statements ]:
P201-P308 + P313

[ Hazard Codes ]:
F:Flammable

[ Risk Phrases ]:
R11;R45;R63

[ Safety Phrases ]:
53-45-36/37-16-36/37/39

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ RTECS ]:
QJ6440000

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Related Compounds

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