PNPP

Names

[ Name ]:
PNPP

[Synonym ]:
4-Nitrophenyl dihydrogen phosphate
disodium,(4-nitrophenyl) phosphate
4-Nitrophenylphosphoric Acid Disodium Salt
4-Nitrophenyl phosphate disodium salt
Phosphoric acid mono(4-nitrophenyl) ester
4-Nitrophenyl phosphate disodium salt hexahydrate
p-Nitrophenyl Phosphate Liquid Substrate System
p-nitro-Phenol dihydrogen phosphate
4-Nitrophenyl phosphate,disodium salt,hexahydrate
para-Nitrophenylphosphate
EINECS 224-246-5
MFCD00007319
Phosphoric acid mono(p-nitrophenyl) ester
Phosphoric acid, mono(4-nitrophenyl) ester
Sodium 4-nitrophenyl phosphate
PNPP

Biological Activity

[Description]:

PNPP is a non-proteinaceous chromogenic substrate for alkaline and acid phosphatases used in ELISA and conventional spectrophotometric assays.

[Related Catalog]:

Research Areas >> Others

Dye Reagents

[In Vitro]

Phosphatases catalyze the hydrolysis of PNPP liberating inorganic phosphate and the conjugate base of para-nitrophenol (pNP). The resulting phenolate is yellow, with a maximal absorption at 405 nm[1].

[Related Small Molecules]

Chemical & Physical Properties

[ Density]:
1.7±0.1 g/cm3

[ Boiling Point ]:
457.8±47.0 °C at 760 mmHg

[ Melting Point ]:
>300 °C(lit.)

[ Molecular Formula ]:
C₆H₄NNa₂O₆P

[ Molecular Weight ]:
219.089

[ Flash Point ]:
230.7±29.3 °C

[ Exact Mass ]:
218.993271

[ PSA ]:
128.05000

[ LogP ]:
0.37

[ Vapour Pressure ]:
0.0±1.2 mmHg at 25°C

[ Index of Refraction ]:
1.617

[ Storage condition ]:
2-8°C

[ Stability ]:
Stable, but may be light sensitive. Incompatible with strong acids, strong bases, strong oxidizing agents.

[ Water Solubility ]:
H2O: 1 tablet/3 mL 25 mM, clear, light yellow

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS05, GHS08

[ Signal Word ]:
Danger

[ Hazard Statements ]:
H290-H315-H318-H373

[ Precautionary Statements ]:
P260-P280-P305 + P351 + P338 + P310

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
S26-S36-S24/25-S23

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

[ HS Code ]:
2919900090

Synthetic Route

Click to get detail synthetic route

Precursor & DownStream

Precursor

DownStream

Customs

[ HS Code ]: 2919900090

[ Summary ]:
2919900090 other phosphoric esters and their salts, including lactophosphates; their halogenated, sulphonated, nitrated or nitrosated derivatives。supervision conditions:AB(certificate of inspection for goods inward,certificate of inspection for goods outward)。VAT:17.0%。tax rebate rate:9.0%。MFN tariff:6.5%。general tariff:30.0%

Articles

Enhancement of enzymatic colorimetric response by silver island films on high throughput screening microplates.

J. Immunol. Methods 411 , 43-9, (2014)

In this study, we report the use of an enzyme-based hybrid platform, which is comprised of silver island films, enzymes (HRP and AP) and high-throughput screening (HTS) microplates, to enhance the col...

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Biotechnol. Bioeng. 112(2) , 308-21, (2015)

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Sprayed cells and polyelectrolyte films for biomaterial functionalization: the influence of physical PLL-PGA film treatments on dental pulp cell behavior.

Macromol. Biosci. 14(12) , 1771-82, (2014)

Further development of biomaterials is expected as advanced therapeutic products must be compliant to good manufacturing practice regulations. A spraying method for building-up polyelectrolyte films f...

Related Compounds

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