Resorufin benzyl ether

Names

[ Name ]:
Resorufin benzyl ether

[Synonym ]:
BENZYLOXYRESORUFIN
7-BENZYLOXYRESORUFIN
RESORUFIN BENZYL ETHER
7-Benzyloxyphenoxazone
7-BENZYLOXY-3H-PHENOXAZIN-3-ONE
MFCD00171613
7-Benzyloxy-3H-phenoxazin-3-one,7-Benzyloxyresorufin,O7-Benzylresorufin
Resorufin benzyl ether,7-Benzyloxy-3H-phenoxazin-3-one,7-Benzyloxyresorufin,O7-Benzylresorufin
Benzyloxyresorfin

Biological Activity

[Description]:

Resorufin benzyl ether (BzRes), a fluorogenic enzyme substrate, can be used to detect CYP3A4 enzyme activity. Resorufin benzyl ether modified with a recognizing moiety boronate, can be used for ONOO- detection via a self-immolation mechanism. Ex/Em=530-570 nm/590 nm[1][2][3].

[Related Catalog]:

Research Areas >> Others

[In Vitro]

CYP3A4 活性测定[2]: 1. 准备反应溶液。 a. 标准原液含有 1 mM Resorufin benzyl ether，以其为荧光底物。将 5mg Resorufin benzyl ether 溶解在 1mL 2% w/v 的 Poloxamer 188 (HY-D1005A)、500 mL Dimethyl sulfoxide (HY-Y0320) 和 3.5 mL 乙腈的混合物中。b. 新鲜配制 CYP3A4 酶液。将 5mL 1mM 酶原液与 995 mL 缓冲液稀释，得到 5 nM 酶溶液。2. 测定 CYP3A4 活性 a. 在 96 孔板中反应。在每个孔中加入 99 mL 缓冲混合液，1 mL 1 mM Resorufin benzyl ether。调整至最终浓度 5 mM。 b. 在 37℃ 孵育 30 分钟之前，转移 100 mL 5 nM 酶液。 c. 在激发波长 (lex=570 nm) 和发射波长 (lem=590 nm) 荧光检测下进行酶活性测定。 d. 影响 CYP3A4 活性测定的因素。如缓冲液 (磷酸盐、Tris-HCl 缓冲液)、缓冲液浓度 (50-200 mM)、培养时间 (0-50 min)。 CYP3A4 代谢活性测定[3]： 1. 加入 CYP3A4 酶至最终浓度为 5 pmol/孔。 2. 每次反应使用 50 pM 底物和 200 mM 磷酸钾缓冲液。< br / >3. 在测量代谢物荧光之前，用 BzRes 孵育 45 分钟。激发波长 (Ex) 为 530 nm，发射波长 (Em) 为590 nm。 4. 将全强度提取物的浓度指定为 100% (在最终测定量中稀释为 1:4)。 5. 以 1:3 重复稀释两次 100% 提取物。计算荧光值的均值和标准差。

[References]

[1]. Ji X, et al. Regulating the activity of boronate moiety to construct fluorescent probes for the detection of ONOO-in vitro and in vivo. Anal Methods. 2022 Dec 15;14(48):5027-5033.

[2]. Nuchtavorn N, et al. Paper-based sol-gel thin films immobilized cytochrome P450 for enzyme activity measurement. Anal Chim Acta. 2020 Feb 15;1098:86-93.

[3]. Yale SH, et al. Analysis of the inhibitory potential of Ginkgo biloba, Echinacea purpurea, and Serenoa repens on the metabolic activity of cytochrome P450 3A4, 2D6, and 2C9. J Altern Complement Med. 2005 Jun;11(3):433-9.

Chemical & Physical Properties

[ Density]:
1.26 g/cm3

[ Boiling Point ]:
479.9ºC at 760 mmHg

[ Molecular Formula ]:
C₁₉H₁₃NO₃

[ Molecular Weight ]:
303.31100

[ Exact Mass ]:
303.09000

[ PSA ]:
52.33000

[ LogP ]:
3.87180

[ Storage condition ]:
2-8°C

MSDS

Click to get detail MSDS

Safety Information

[ Symbol ]:

GHS07

[ Signal Word ]:
Warning

[ Hazard Statements ]:
H315-H319-H335

[ Precautionary Statements ]:
P261-P305 + P351 + P338

[ Personal Protective Equipment ]:
dust mask type N95 (US);Eyeshields;Gloves

[ Hazard Codes ]:
Xi: Irritant;

[ Risk Phrases ]:
R36/37/38

[ Safety Phrases ]:
26-36

[ RIDADR ]:
NONH for all modes of transport

Articles

Modulation of cytochrome P450 2A5 activity by lipopolysaccharide: low-dose effects and non-monotonic dose-response relationship.

PLoS ONE 10(1) , e0117842, (2015)

Mouse cytochrome P450 (CYP) 2A5 is induced by inflammatory conditions and infectious diseases that down-regulate the expression and activity of most other CYP isoforms. Enhanced oxidative stress and n...

Inhibition of cytochrome P450 2B4 by environmentally persistent free radical-containing particulate matter.

Biochem. Pharmacol. 95 , 126-32, (2015)

Combustion processes generate particulate matter (PM) that can affect human health. The presence of redox-active metals and aromatic hydrocarbons in the post-combustion regions results in the formatio...

Inhibition against mosquito cytochrome P450 enzymes by rhinacanthin-A, -B, and -C elicits synergism on cypermethrin cytotoxicity in Spodoptera frugiperda cells.

J. Med. Entomol. 49(5) , 993-1000, (2012)

Rhinacanthus nasutus (Acanthaceae) is a shrub reported to contain insecticidal activities. The current study was conducted to determine whether R. nasutus constituents could inhibit benzyloxyresorufin...

Related Compounds

The content on this webpage is sourced from various professional data sources. If you have any questions or concerns regarding the content, please feel free to contact service1@chemsrc.com.