ec 4.1.3.3

Names

[ Name ]:
ec 4.1.3.3

[Synonym ]:
MFCD00131656

Biological Activity

[Description]:

N-Acetylneuraminic acid lyase (NAL) is a class I aldolase, is often used in biochemical studies. N-Acetylneuraminic acid lyase catalyzes the reversible condensation of pyruvate with N-acetyl-d-mannosamine (ManNAc) to yield the sialic acid N-acetylneuraminic acid (Neu5Ac)[1].

[Related Catalog]:

Research Areas >> Others

[References]

[1]. Daniels AD, et al. Reaction mechanism of N-acetylneuraminic acid lyase revealed by a combination of crystallography, QM/MM simulation, and mutagenesis. ACS Chem Biol. 2014 Apr 18;9(4):1025-32.

Chemical & Physical Properties

[ Density]:
1.000 g/cm3

[ Molecular Formula ]:
C₂₃H₄₂O₅

[ Molecular Weight ]:
398.583

[ Appearance of Characters ]:
powder | slightly yellow

[ Storage condition ]:
−20°C

Safety Information

[ Personal Protective Equipment ]:
Eyeshields;Gloves;half-mask respirator (US);multi-purpose combination respirator cartridge (US)

[ Hazard Codes ]:
Xi

[ Risk Phrases ]:
36/37/38

[ Safety Phrases ]:
23-24/25-60-37-26-22

[ RIDADR ]:
NONH for all modes of transport

[ WGK Germany ]:
3

Articles

Chemistry, metabolism, and biological functions of sialic acids.

Adv. Carbohydr. Chem. Biochem. 40 , 131, (1982)

Structural basis for substrate specificity and mechanism of N-acetyl-D-neuraminic acid lyase from Pasteurella multocida.

Biochemistry 52(47) , 8570-9, (2013)

N-Acetylneuraminate lyases (NALs) or sialic acid aldolases catalyze the reversible aldol cleavage of N-acetylneuraminic acid (Neu5Ac, the most common form of sialic acid) to form pyruvate and N-acetyl...

Structural insights into the recovery of aldolase activity in N-acetylneuraminic acid lyase by replacement of the catalytically active lysine with γ-thialysine by using a chemical mutagenesis strategy.

ChemBioChem. 14(4) , 474-81, (2013)

Chemical modification has been used to introduce the unnatural amino acid γ-thialysine in place of the catalytically important Lys165 in the enzyme N-acetylneuraminic acid lyase (NAL). The Staphylococ...

Related Compounds

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