130926-94-2
130926-94-2 structure
- Name: Mag-indo-1/AM
- Chemical Name: acetyloxymethyl 2-[4-[bis[2-oxo-2-(2-oxopropoxymethoxy)ethyl]amino]phenyl]-1H-indole-6-carboxylate
- CAS Number: 130926-94-2
- Molecular Formula: C30H32N2O12
- Molecular Weight: 612.58100
- Catalog: Research Areas Others
- Create Date: 2018-05-18 08:00:00
- Modify Date: 2024-01-14 10:16:51
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Mag-indo-1/AM is a cell-permeable fluorescent indicator for Mg2+ and also for Ca2+ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg2+ and Ca2+ concentrations[1][2].
| Name | acetyloxymethyl 2-[4-[bis[2-oxo-2-(2-oxopropoxymethoxy)ethyl]amino]phenyl]-1H-indole-6-carboxylate |
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| Synonyms |
Indo 1-AM analog
Mag-Indo 1-AM Mag-Indo-1AM |
| Description | Mag-indo-1/AM is a cell-permeable fluorescent indicator for Mg2+ and also for Ca2+ (Ex=340-390 nm, Em=410-490 nm). Mag-indo-1/AM can be used to determine intracellular Mg2+ and Ca2+ concentrations[1][2]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Measurement of intracellular Ca2+[1]: 1. Incubate 3 mL cell suspension with 10 µM Mag-indo-1/AM for 15 min at 30℃. 2. Centrifuge the cells for 1 min at 500 rpm and resuspend in 5 mL isolation buffer (containing 1 mM CaCl2). 3. Add 1 mM probenecid to the cell suspension to prevent dye leakage from the cytosol and store the cells (Mag-indo-1/AM-loaded cells) at room temperature until use. 4. Incubate the Mag-indo-1/AM-loaded cells at room temperature for 2 h, shake gently at 37℃ for 1.5 h, centrifuge the pellet, resuspend and store at room temperature. 6. Place a small portion of the loaded cells in an experimental chamber which is mounted on the stage of an inverted microscope. 7. Measure fluorescence of Mag-indo-1/AM (Ex=340-390 nm, Em=410 and 490 nm (the peak emissions of the Ca2+ bound and Ca2+ free forms of the indicator)). Fluorescence of the whole cell is collected on line by a photon counting system. |
| References |
| Density | 1.335g/cm3 |
|---|---|
| Boiling Point | 789.9ºC at 760mmHg |
| Molecular Formula | C30H32N2O12 |
| Molecular Weight | 612.58100 |
| Flash Point | 431.5ºC |
| Exact Mass | 612.19600 |
| PSA | 176.83000 |
| LogP | 2.53130 |
| Vapour Pressure | 6.32E-25mmHg at 25°C |
| Index of Refraction | 1.585 |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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| RIDADR | NONH for all modes of transport |