1566-81-0
1566-81-0 structure
- Name: NS1652
- Chemical Name: NS1652
- CAS Number: 1566-81-0
- Molecular Formula: C15H11F3N2O3
- Molecular Weight: 324.255
- Catalog: Signaling Pathways Membrane Transporter/Ion Channel Chloride Channel
- Create Date: 2018-02-03 10:31:11
- Modify Date: 2024-01-11 13:23:38
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NS1652 is a reversible anion conductance inhibitor, blocks chloride channel, with an IC50 of 1.6 μM in human and mouse red blood cells.
| Name | NS1652 |
|---|---|
| Synonyms |
2-({[3-(Trifluoromethyl)phenyl]carbamoyl}amino)benzoic acid
NS-1652 9B8X1YC8U2 Benzoic acid, 2-[[[[3-(trifluoromethyl)phenyl]amino]carbonyl]amino]- |
| Description | NS1652 is a reversible anion conductance inhibitor, blocks chloride channel, with an IC50 of 1.6 μM in human and mouse red blood cells. |
|---|---|
| Related Catalog | |
| Target |
IC50: 1.6 μM (chloride channel, human and mouse red blood cell)[1] |
| In Vitro | NS1652 potently inhibits the chloride conductance (IC50, 1.6 μM) in human and mouse red blood cells, but only weakly inhibits VRAC (IC50, 125 μM) in HEK293 cells. NS1652 markedly blocks the NO production with an IC50 of 3.1 μM in BV2 cells. NS1652 also down-regulates iNOS expression at 3 μM, and completely abolishes at 10 μM in BV2 cells[1]. NS1652 (0, 1.0, 3.3, 10, and 20 μM) causes increasing hyperpolarization due to inhibition of the chloride conductance in normal erythrocytes. NS1652 lowers the net KCl loss from deoxygenated sickle cells from about 12 mM cells/h to about 4 mM cells/h. NS1652 (20 μM) completely and reversiblely inhibits the red cell Cl-conductance[2]. |
| In Vivo | NS1652 (50 mg/kg, i.v.) blocks murine erythrocyte Cl- conductance by >90% in mice[2]. |
| Animal Admin | NS1652 is suspended in a carrying vehicle, cremophore (pig-40 hydrogenated castor oil), at a concentration of 5 mg/mL. At time zero, an amount corresponding to 1% of animal weight (about 250 μL of suspension) is injected into mice though the tail veins (NMRI strain, 5-6 weeks). At several time intervals after the injection, the mice are decapitated and the blood collected is collected and centrifuged for 60 seconds. The plasma is removed by aspiration and the packed cells are stored on ice until use. Immediately before measurement, the packed cells are resuspended in 1 volume of ice-cold experimental medium and centrifuged for 30 seconds. A total of 100 μL of packed cells are then immediately transferred to 3 mL medium, and CCCP and valinomycin added. The blood samples are analyzed in random order with respect to the time of decapitation[2]. |
| References |
| Density | 1.5±0.1 g/cm3 |
|---|---|
| Boiling Point | 355.9±42.0 °C at 760 mmHg |
| Molecular Formula | C15H11F3N2O3 |
| Molecular Weight | 324.255 |
| Flash Point | 169.1±27.9 °C |
| Exact Mass | 324.072174 |
| LogP | 5.01 |
| Vapour Pressure | 0.0±0.8 mmHg at 25°C |
| Index of Refraction | 1.632 |
| Storage condition | 2-8℃ |