171783-05-4
171783-05-4 structure
- Name: Crosstide
- Chemical Name: Crosstide
- CAS Number: 171783-05-4
- Molecular Formula: C48H77N17O17
- Molecular Weight: 1164.229
- Catalog: Peptides
- Create Date: 2018-01-28 06:18:53
- Modify Date: 2024-01-02 09:01:58
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Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt.
| Name | Crosstide |
|---|---|
| Synonyms | MFCD03787963 |
| Description | Crosstide is a peptide analog of glycogen synthase kinase α/β fusion protein sequence which is a substrate for Akt. |
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| Related Catalog | |
| Target |
Akt |
| In Vitro | 16HBE14o− cells exposed to RV39 demonstrate a crosstide kinase activity in vitro. Serine phosphorylation of crosstide is confirmed by immunoblotting, and phosphorylation is blocked by PP2 but not by PP3[1]. The wildtype GST-AKT2 shows significant phosphoryl transferase activity towards crosstide, reaching an initial velocity of 16 pmol phosphate/min/μg enzyme. The mutant GST-AKT2T/E,S/D displayed an initial velocity of 85 pmol phosphate/min/μg kinase for phosphorylation of Crosstide, corresponding to a 5-fold increase compared to the wildtype enzyme[2]. |
| Kinase Assay | After serum deprivation for 24 h, cells are incubated with digoxigenin-labeled sham protein or digoxigenin-labeled RV39 at an MOI of 1.0 for 10 min. Cell homogenates are immunoprecipitated with mouse anti-digoxigenin antibody and precipitates incubated with Crosstide and [γ-32P]ATP. Crosstide is a glycogen synthase kinase α/β fusion protein sequence (GRPRTSSFAEG) which is a substrate for Akt. Samples are processed for autoradiography and immunoblotting using rabbit anti-phospho-Tyr416 Src, mouse anti-Src (clone GD11), rabbit anti-phospho-Ser473, or rabbit anti-Akt. |
| References |
| Density | 1.6±0.1 g/cm3 |
|---|---|
| Molecular Formula | C48H77N17O17 |
| Molecular Weight | 1164.229 |
| Exact Mass | 1163.568359 |
| LogP | -4.77 |
| Index of Refraction | 1.678 |
| Storage condition | 2-8℃ |
| Personal Protective Equipment | Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter |
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| RIDADR | NONH for all modes of transport |