794458-56-3
794458-56-3 structure
- Name: 360A
- Chemical Name: 360 A
- CAS Number: 794458-56-3
- Molecular Formula: C27H23N5O2
- Molecular Weight: 449.5
- Catalog: Signaling Pathways Cell Cycle/DNA Damage G-quadruplex
- Create Date: 2018-10-16 11:26:16
- Modify Date: 2024-01-12 12:20:55
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360A is a selective stabilizer of G-quadruplex, and also inhibits telomerase activity with an IC50 of 300 nM for telomerase in TRAP-G4 assay.
| Name | 360 A |
|---|---|
| Synonyms | 360A |
| Description | 360A is a selective stabilizer of G-quadruplex, and also inhibits telomerase activity with an IC50 of 300 nM for telomerase in TRAP-G4 assay. |
|---|---|
| Related Catalog | |
| Target |
IC50: 300 nM (Telomerase)[1] G-quadruplex[1] |
| In Vitro | 360A inhibits telomerase activity and stabilizes G-quadruplex, with an IC50 of 300 nM for telomerase in TRAP-G4 assay. 360A reduces the viability of glioma cell lines, such as T98G, CB193, U118-MG, SAOS-2 and Primary astrocytes, with IC50s of 4.8 ± 1.1 μM, 3.9 ± 0.4 μM, 8.4 ± 0.5 μM, >15 μM and 17.4 ± 1.2 μM, respectively[1]. 360A causes Rad51-dependent telomere aberrations preferentially involving the lagging strand telomeres, including telomere losses or telomere doublets, and induces DNA-PKcs-dependent sister telomere fusions[2]. |
| Cell Assay | The cell proliferation reagent WST-1 assay is performed. In brief, cells are seeded at various densities, depending on cell type (0.25-4 × 103 cells/well in 100 μL complete medium), in 96-well culture plates and treated with various concentrations (0.1-20 μM) of 360A or the corresponding concentrations of DMSO (control wells) for 3 or 7 days at 37°C in an atmosphere containing 5% CO2. For 7-day assays, the medium is changed on day 3. Experiments are performed in triplicate[1]. |
| References |
| Molecular Formula | C27H23N5O2 |
|---|---|
| Molecular Weight | 449.5 |
| Storage condition | -20°C |