1220751-16-5
1220751-16-5 structure
- Name: TAT-DEF-Elk-1
- Chemical Name: TAT-DEF-Elk-1
- CAS Number: 1220751-16-5
- Molecular Formula: C155H259N57O40
- Molecular Weight: 3561.07
- Catalog: Research Areas Neurological Disease
- Create Date: 2020-06-01 11:49:16
- Modify Date: 2025-08-25 16:43:05
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TAT-DEF-Elk-1 (TDE) is a cell-penetrating peptide inhibitor of Elk-1, mimics and specifically interferes with the DEF domain of Elk-1. TAT-DEF-Elk-1 blocks Elk-1 phosphorylation and prevents Elk-1 nuclear translocation without interfering with ERK nor MSK1 activation. TAT-DEF-Elk-1 is a useful tool to analyze the role of Elk-1 in this process during the development of neuronal plasticity[1].
| Name | TAT-DEF-Elk-1 |
|---|
| Description | TAT-DEF-Elk-1 (TDE) is a cell-penetrating peptide inhibitor of Elk-1, mimics and specifically interferes with the DEF domain of Elk-1. TAT-DEF-Elk-1 blocks Elk-1 phosphorylation and prevents Elk-1 nuclear translocation without interfering with ERK nor MSK1 activation. TAT-DEF-Elk-1 is a useful tool to analyze the role of Elk-1 in this process during the development of neuronal plasticity[1]. |
|---|---|
| Related Catalog | |
| Target |
IC50: Elk-1[1] |
| In Vitro | Elk-1 phosphorylation on Ser383/389 has a dual function and triggers both Elk-1 nuclear translocation and SRE-dependent gene expression[1]. TAT-DEF-Elk-1 (5-10 μM; 1 hour) specifically inhibits glutamate-induced elk-1 activation and does not interfer with ERK, MSK-1, or CREB phosphorylation[1]. TAT-DEF-Elk-1 (5 μM; 2 hour) treatment shows a significant inhibition of c-Fos, Zif268 and JunB, but has no effects on c-Jun expression[1]. Western Blot Analysis[1] Cell Line: Neurons Concentration: 5 μM; 10 μM Incubation Time: 1 hour Result: Decreased Elk-1 expression and had no effects on ERK, MSK-1, or CREB phosphorylation. RT-PCR[1] Cell Line: Primary striatal neurons Concentration: 5 μM Incubation Time: 2 hour Result: Decreased c-Fos, Zif268 and JunB mRNA level but did not effect c-Jun. |
| In Vivo | TAT-DEF-Elk-1 (intraperitoneal injection; 1mg/kg; daily; 14 days) reflects antidepressant efficacy in mice, it decreases immobility similar to the reference antidepressants fluoxetine and desipramine (DMI)[2]. Animal Model: C57Bl6 mice (3-6 months old males) are subjected to social defeat stress[2] Dosage: 1mg/kg; Administration: Intraperitoneal injection; daily; 14 days Result: Reversed social-defeat induced decrease of hippocampal Bdnf expression by repeated TDE administration. |
| References |
| Molecular Formula | C155H259N57O40 |
|---|---|
| Molecular Weight | 3561.07 |