217190-17-5
217190-17-5 structure
- Name: BODIPY FL SSE
- Chemical Name: BODIPY FL SSE
- CAS Number: 217190-17-5
- Molecular Formula: C18H17BF2N3NaO7S
- Molecular Weight: 491.21
- Catalog: Research Areas Others
- Create Date: 2022-10-13 08:17:29
- Modify Date: 2024-04-06 09:46:10
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BODIPY FL SSE is a potent fluorescent dye. BODIPY FL SSE is used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. BODIPY FL SSE can reactive with primary amines on biomolecules to emit green fluorescence. (λex=502 nm, λem=511 nm)[1][2].
| Name | BODIPY FL SSE |
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| Description | BODIPY FL SSE is a potent fluorescent dye. BODIPY FL SSE is used to label the primary amines (R-NH2) of proteins, amine-modified oligonucleotides, and other amine-containing molecules. BODIPY FL SSE can reactive with primary amines on biomolecules to emit green fluorescence. (λex=502 nm, λem=511 nm)[1][2]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). BODIPY FL SSE assay (to quantify GAPDH)[1]: 1.Make a 2 mg/ mL stock of GAPDH (MW 37 kDa) in PBS (pH 7.4). 2.Add 54 nM of BODIPY-FL-SSE to 1 mL of the 2 mg/ mL (54 μM) GAPDH solution. Incubate the mixture for 4 h on ice. 3.Dialyze the reaction mixture against 500 ml of PBS, pH 7.4, in a 10,000 MW-cutoff dialysis cassette for 1 h at 4 °C. Change the buffer and dialyze again overnight. Change the dialysis buffer once more the following morning and dialyze further for 1 h. 4.Run the dialyzed sample through a Zeba desalting column at 1000 g for 2 min. 5.Analyze the BD-GAPDH spectrophotometrically in a quartz cuvette. Set the wavelength scan to 250-700 nm. Blank the instrument against PBS and add the BD-GAPDH to the cuvette. |
| References |
| Molecular Formula | C18H17BF2N3NaO7S |
|---|---|
| Molecular Weight | 491.21 |