2755154-93-7

2755154-93-7 structure

Name: Cyanine3 maleimide tetrafluoroborate
Chemical Name: Cyanine3 maleimide tetrafluoroborate
CAS Number: 2755154-93-7
Molecular Formula: C₃₆H₄₃BF₄N₄O₃₂-
Molecular Weight: 666.56
Catalog: Research Areas Others
Create Date: 2022-11-21 17:14:31
Modify Date: 2024-07-19 17:44:06
Cyanine3 maleimide tetrafluoroborate is a specific labeling reagent for sulfhydryl groups with the λex of 550 nm and λem of 580 nm. Cyanine3 maleimide tetrafluoroborate can be used for protein and peptide labeling[1][2].

Name	Cyanine3 maleimide tetrafluoroborate

Description	Cyanine3 maleimide tetrafluoroborate is a specific labeling reagent for sulfhydryl groups with the λex of 550 nm and λem of 580 nm. Cyanine3 maleimide tetrafluoroborate can be used for protein and peptide labeling[1][2].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).Labeling of Protein:1.The protein concentration was adjusted to 1 mg/mL for 100 μL.2. A 100 μL aliquot of the protein solution was mixed with Cyanine3 maleimide tetrafluoroborate (8 μg) in DMSO (2 μL).3. Incubated overnight at 4 °C in the dark.4. Labeled proteins were desalted using a G25 desalting column to remove excessive Cyanine3 maleimide tetrafluoroborate.5. Fluorescence intensities (FI) of labeled proteins were measured at excitation and emission wavelengths of 550 nm and 570 nm, respectively.
References	[1]. Zartner L, et al. Membrane protein channels equipped with a cleavable linker for inducing catalysis inside nanocompartments. J Mater Chem B. 2021 Nov 10;9(43):9012-9022. [2]. He J, et al. The effect of meat processing methods on changes in disulfide bonding and alteration of protein structures: impact on protein digestion products. RSC Adv. 2018 May 15;8(31):17595-17605.

Molecular Formula	C₃₆H₄₃BF₄N₄O₃₂-
Molecular Weight	666.56