| Name | PFB-FDG |
|---|
| Description | PFB-FDG is a non-fluorescent galactosidase substrate that can be hydrolysed to green fluorescent PFB-F (Ex=485 nm, Em=535 nm). PFB-FDG can be used for the determination of β-galactosidase activity[1]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Measurement of β-galactosidase activity[1]: 1. Incubate 2 × 105 cells with 50 µg/mL of PFB-FDG in serum-free DMEM containing 25 mM Hepes (pH 7.4) for 2 h at 37℃. (Mononuclear cells for example, different cell concentrations vary) 2. Wash cells once with ice-cold PBS. 3. Analyze cells by flow cytometry (Ex=485 nm, Em=535 nm). |
| References |
| Molecular Formula | C39H32F5NO16 |
|---|---|
| Molecular Weight | 865.66 |