2183473-57-4
2183473-57-4 structure
- Name: Cy3 methyltetrazine
- Chemical Name: Cy3 methyltetrazine
- CAS Number: 2183473-57-4
- Molecular Formula: C40H46BF4N7O
- Molecular Weight: 727.64
- Catalog: Research Areas Others
- Create Date: 2022-12-28 20:14:06
- Modify Date: 2024-04-05 21:13:31
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Cy3 methyltetrazine (TZ-Cy3) is a click chemistry reagent with methyltetrazine building blocks that is highly reactive towards cyclooctene. Cy3 methyltetrazine is also a tetrazine-modified fluorescent probe that can be used to analyze protein phosphorylation in solution and living cells[1].
| Name | Cy3 methyltetrazine |
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| Description | Cy3 methyltetrazine (TZ-Cy3) is a click chemistry reagent with methyltetrazine building blocks that is highly reactive towards cyclooctene. Cy3 methyltetrazine is also a tetrazine-modified fluorescent probe that can be used to analyze protein phosphorylation in solution and living cells[1]. |
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| Related Catalog | |
| In Vitro | Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Confocal imaging of phosphorylation in cells: 1. Culture H1299 cells in a coverglass-bottom imaging dish for 24 h (take H1299 cells as an example). 2. Incubate H1299 cells with different concentrations of ATP-NB (a ATP analogue functionalized by norbornene, which can penetrate cells and efficiently phosphorylate proteins in living cells) for 1 h. 3. Add Cy3 methyltetrazine to label the phosphorylated protein with fluorescence. 4. Incubate with fresh medium for 1 h. 5. Wash the cells twice with PBS to release the free Cy3 methyltetrazine probe. 6.Incubate the cells with 4% paraformaldehyde for 15 min and with 0.1% Triton X-100 solution at room temperature for 10 min. 7. Wash cells 4 times with PBS. 8. Use a confocal microscopy imaging system to obtain the high-resolution images of cells (Ex=488 nm; Em=561 nm). |
| Molecular Formula | C40H46BF4N7O |
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| Molecular Weight | 727.64 |