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94367-21-2

94367-21-2 structure

94367-21-2 structure

Name: Suc-Leu-Leu-Val-Tyr-AMC
Chemical Name: succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin
CAS Number: 94367-21-2
Molecular Formula: C₄₀H₅₃N₅O₁₀
Molecular Weight: 763.87600
Catalog: Biochemical Amino acids and their derivatives Tyrosine derivatives
Create Date: 2018-12-24 13:22:47
Modify Date: 2026-07-01 12:22:18
Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate. Sequence: Suc-Leu-Leu-Val-Tyr.

Name	succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumarin
Synonyms	SUC-LEU-LEU-VAL-TYR-MCA PROTEASOME SUBSTRATE III (FLUOROGENIC) CHYMOTRYPSIN SUBSTRATE III, FLUOROGENIC ; SUC-LEU-LEU-VAL-TYR-AMC succinyl-leucyl-leucyl-valyl-tyrosyl-methylcouminamide Calpain Substrate Suc-LLVY-AMC, fluorogenic substrate suc-llvy-amc SUC-LEU-LEU-VAL-TYR-7-AMINO-4-METHYLCOUMIN 4-[[1-[[1-[[(1S)-1-[[(1S)-1-(4-hydroxybenzyl)-2-keto-2-[(2-keto-4-methyl-chromen-7-yl)amino]ethyl]cbamoyl]-2-methyl-propyl]cbamoyl]-3-methyl-butyl]cbamoyl]-3-methyl-butyl]amino]-4-keto-butyric acid Suc-Leu-Leu-Val-Tyr-AMC(TFA) n-succinyl-leu-leu-val-tyr 7-amido-4-methylcoumarin SUC-LLVY-AMC suc-leu-leu-val-tyr-mca N-SUCCINYL-LEU-LEU-VAL-TYR 7-AMIDO-4-METHYLCOUMIN suc-leu-leu-val-tyr-7-amino-4-methylcoumarin CALPAIN SUBSTRATE (FLUOROGENIC) chymotrypsin substrate iii,fluorogenic SUCCINYL-L-LEUCYL-L-LEUCYL-L-VALYL-L-TYROSINE-4-METHYLCOUMINYL-7-AMIDE N-SUCCINYL-LEU-LEU-VAL-TYR 7-AMIDO-4-*ME THYLCOUMI N-Succinyl-l-leucyl-l-leucyl-l-valyl-l-tyrosine-4-methylcoumyl-7-amidefluorogenicsubstrateforchymotryspin SUCCINYL-L-LEUCYL-L-LEUCYL-L-VALYL-L-TYROSINE 4-METHYLCOUMYL-7-AMIDE MFCD00080248 z-llvy-amc suc-leu-leu-val-tyr-amc Suc-Leu-Leu-Val-Tyr-AMC, Fluorogenic substrate of calpains and 20S proteasome Z-LLVY-AMC CHYMOTRYPSIN SUBSTRATE III, FLUOROGENIC N-SUCCINYL-LEU-LEU-VAL-TYR-7-AMINO-4-METHYLCOUMIN AMC SUC-LEU-LEU-VAL-TYR-AMC //BP L-Tyrosinamide, N-(3-cboxy-1-oxopropyl)-L-leucyl-L-leucyl-L-valyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl) SUC-LEU-LEU-VAL-TYR-AMC succinyl-Leu-Leu-Val-Tyr-7-amino-4-methylcoumin 4-[[1-[[1-[[(2S)-1-[[(2S)-3-(4-hydroxyphenyl)-1-[(4-methyl-2-oxo-chromen-7-yl)amino]-1-oxo-propan-2-yl]amino]-3-methyl-1-oxo-butan-2-yl]amino]-4-methyl-1-oxo-pentan-2-yl]amino]-4-methyl-1-oxo-pentan-2-yl]amino]-4-oxo-butanoic acid N-SUCCINYL-L-LEUCYL-L-LEUCYL-L-VALYL-L-TYROSINE-4-METHYLCOUMYL-7-AMIDE Felypressin Impurity 8 4-[[1-[[1-[[(2S)-1-[[(2S)-3-(4-hydroxyphenyl)-1-[(4-methyl-2-oxochromen-7-yl)amino]-1-oxopropan-2-yl]amino]-3-methyl-1-oxobutan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-4-methyl-1-oxopentan-2-yl]amino]-4-oxobutanoic acid PROTEASOME 20S, (SUC-LEU-LEU-VAL-TYR-AMC)

Description	Suc-Leu-Leu-Val-Tyr-AMC is a fluorogenic substrate. Sequence: Suc-Leu-Leu-Val-Tyr.
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Peptides Research Areas >> Others
In Vitro	Suc-Leu-Leu-Val-Tyr-AMC (Suc-LLVY) is a membrane-permeable calpain-specific fluorogenic substrate, pteolytic hydrolysis of the peptidyl-7-amino bond liberates the highly fluorescent 7-amino-4-methylcoumarin (AMC) moiety[1]. The effectof TGF-β on hydrolysis of these substrates (e.g Suc-Leu-Leu-Val-Tyr-AMC) are assessed. Biliary epithelial H69 cells are incubated with 10, 1, 0.1, or 0 ng/mL TGF-β for 24 h. Substrate hydrolysis is then fluorometrically assessed in cytosolic extracts. Basal activity is 1.12, 8.33, and 14.52 nmol AMC/mg protein/min for suc-LLVY-AMC, z-LLE-AMC, and z-LLL-AMC hydrolysis, respectively[2].
Kinase Assay	Immunoprecipitation is carried out for the two sets of samples, using the same amount of protein. The 20 S and 26 S proteasome immunoprecipitates are washed with 50 mM Hepes/KOH (pH 7.5), and 50 mM Hepes/KOH (pH 7.5) containing 2 mMATP, respectively, prior to the determination of peptidase activity using 50 μM suc-Leu- Leu-Val-Tyr-AMC as substrate in these buffers[3].
References	[1]. Roberta L. Debiasi, et al. Reovirus-Induced Apoptosis Is Preceded by Increased Cellular Calpain Activity and Is Blocked by Calpain Inhibitors. J Virol. 1999 Jan; 73(1): 695–701. [2]. Tadlock L, et al. Transforming growth factor-beta inhibition of proteasomal activity: a potential mechanism of growth arrest. Am J Physiol Cell Physiol. 2003 Aug;285(2):C277-85. Epub 2003 Mar 19. [3]. Gardner RC, et al. Characterization of peptidyl boronic acid inhibitors of mammalian 20 S and 26 S proteasomes and their inhibition of proteasomes in cultured cells. Biochem J. 2000 Mar, 2:447-54.

Density	1.249g/cm3
Boiling Point	1116.8ºC at 760mmHg
Molecular Formula	C₄₀H₅₃N₅O₁₀
Molecular Weight	763.87600
Flash Point	629.2ºC
Exact Mass	763.37900
PSA	233.24000
LogP	5.17700
Index of Refraction	1.577
Storage condition	−20°C

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