1435488-37-1
1435488-37-1 structure
- Name: xmd17-109
- Chemical Name: 11-cyclopentyl-2-[2-ethoxy-4-[4-(4-methylpiperazin-1-yl)piperidine-1-carbonyl]anilino]-5-methylpyrimido[4,5-b][1,4]benzodiazepin-6-one
- CAS Number: 1435488-37-1
- Molecular Formula: C36H46N8O3
- Molecular Weight: 638.802
- Catalog: Research Areas Cancer
- Create Date: 2017-06-14 01:39:52
- Modify Date: 2024-01-03 02:15:21
-
XMD17-109 is a novel, specific ERK-5 inhibitor, with an IC50 of 162 nM.
| Name | 11-cyclopentyl-2-[2-ethoxy-4-[4-(4-methylpiperazin-1-yl)piperidine-1-carbonyl]anilino]-5-methylpyrimido[4,5-b][1,4]benzodiazepin-6-one |
|---|---|
| Synonyms |
6H-Pyrimido[4,5-b][1,4]benzodiazepin-6-one, 11-cyclopentyl-2-[[2-ethoxy-4-[[4-(4-methyl-1-piperazinyl)-1-piperidinyl]carbonyl]phenyl]amino]-5,11-dihydro-5-methyl-
11-Cyclopentyl-2-[(2-ethoxy-4-{[4-(4-methyl-1-piperazinyl)-1-piperidinyl]carbonyl}phenyl)amino]-5-methyl-5,11-dihydro-6H-pyrimido[4,5-b][1,4]benzodiazepin-6-one xmd17-109 |
| Description | XMD17-109 is a novel, specific ERK-5 inhibitor, with an IC50 of 162 nM. |
|---|---|
| Related Catalog | |
| Target |
ERK5:162 nM (IC50) LRRK2[G2019S]:339 nM (IC50) |
| In Vitro | XMD17-109 (Compound 26) inhibits ERK5 biochemically with an IC50 of 0.162 ± 0.006 μM, and blocks pidermal growth factor induced ERK5 autophosphorylation with an EC50 of 0.09 ± 0.03 μM in cells. XMD17-109 also inhibits LRRK2[G2019S] with an IC50 of 339 nM[1]. XMD17-109 demonstrats low nanomolar cellular activity judged by the significant dose-dependent reduction of mobility shifted phosphorylated ERK5 bands from sorbitol stimulated cells. XMD17-109 completely inhibits the ERK5-mediated AP1 transcriptional activity at 30 μM and has an EC50 of 4.2 μM[2]. |
| Cell Assay | HeLa cells are maintained in DMEM supplemented with 10% FBS, 2 mM l-glutamine, 50 U/mL penicillin G, and 50 μg/mL streptomycin. Before use HeLa cells are serum starved for 16 h in DMEM supplemented with 2 mM l-glutamine, 50 U/mL penicillin G, and 50 μg/mL streptomycin. HeLa cells are then incubated with ERK5-IN-1 at the indicated concentrations for 1 h prior to stimulation with 0.5mol/Lsorbitol for 30 min. Cells are lysed in Triton lysis buffer (50 mM Tris-HCl, pH 7.5, 1 mM EGTA, 1 mM EDTA, 1 mM sodium orthovanadate, 50 mM sodium fluoride, 1 mM sodium pyrophosphate, 0.27mol/Lsucrose, 1 μM microcystin-LR, 1% (v/v) Triton X-100, 0.1% (v/v) 2-mercaptoethanol) and 20 μg of protein loaded per well. Samples are run on 8% polyacrylamide gels using standard methods. Proteins are transferred onto nitrocellulose membranes and specific proteins detected by immunoblotting. |
| References |
| Density | 1.3±0.1 g/cm3 |
|---|---|
| Boiling Point | 831.6±75.0 °C at 760 mmHg |
| Molecular Formula | C36H46N8O3 |
| Molecular Weight | 638.802 |
| Flash Point | 456.8±37.1 °C |
| Exact Mass | 638.369263 |
| PSA | 100.76000 |
| LogP | 2.39 |
| Vapour Pressure | 0.0±3.0 mmHg at 25°C |
| Index of Refraction | 1.640 |
| Storage condition | -20℃ |