325143-98-4
325143-98-4 structure
- Name: Sulfo-NHS-SS-Biotin sodium
- Chemical Name: sodium,1-[3-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethyldisulfanyl]propanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate
- CAS Number: 325143-98-4
- Molecular Formula: C19H27N4NaO9S4
- Molecular Weight: 606.68900
- Catalog: Research Areas Others
- Create Date: 2016-01-13 20:44:20
- Modify Date: 2024-01-10 17:40:12
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Sulfo-NHS-SS-biotin is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin can be used for the labeling and purifying of cell-surface protein[1].
| Name | sodium,1-[3-[2-[5-[(3aS,4S,6aR)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]pentanoylamino]ethyldisulfanyl]propanoyloxy]-2,5-dioxopyrrolidine-3-sulfonate |
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| Synonyms |
Sulfo-NHS-SS-Biotin
3-[[2-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]amino]ethyl]dithio]-propanoic Acid 2,5-dioxo-3-sulfo-1-pyrrolidinyl Ester,Sodium Salt Sulfosuccinimidyl 3-[[2-(Biotinamido)ethyl] dithio]propionate Sodium Salt NHS-SS-BIOTIN Sodium Salt |
| Description | Sulfo-NHS-SS-biotin is a long-chain cleavable and cell-impermeant amine-reactive biotinylation reagent. Sulfo-NHS-SS-biotin can be used for the labeling and purifying of cell-surface protein[1]. |
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| Related Catalog | |
| Target |
IC50: cell-surface protein[1] |
| In Vitro | Sulfo-NHS-SS-biotin is a cell-surface-labeling reagent, it is a negatively charged reagent and does not permeate cell membranes[1]. Sulfo-NHS-SS-biotin reacts with primary amines (NH2), such as lysine side-chains, or the amino-termini of polypeptides[1]. Sulfo-NHS-SS-biotin has a sulfonate group and prevents it from permeating cell membranes. Thr group’s cleavable spacer arm enables initially biotinylated proteins to be released from streptavidin affinity columns[1]. Sulfo-NHS-SS-biotin (1 mg/ml; 15 min) is applied in cells in monolayer culture (washed by ice-cold PBS). The biotinylation reactions are terminated with 100 mM glycine in PBS. After washing with PBS, cell extracts are prepared in RIPA buffer with protease inhibitor cocktail (HY-K0010). Biotinylated membrane proteins are precipitated with streptavidin-sepharose and proteins are eluted with SDS sample buffer[2]. |
| References |
| Molecular Formula | C19H27N4NaO9S4 |
|---|---|
| Molecular Weight | 606.68900 |
| Exact Mass | 606.05600 |
| PSA | 282.37000 |
| LogP | 1.77370 |
| Storage condition | -20°C |