D-2-Hydroxypentanedioic acid disodium salt

Modify Date: 2025-08-25 21:56:27

D-2-Hydroxypentanedioic acid disodium salt Structure
D-2-Hydroxypentanedioic acid disodium salt structure
 Common Name D-2-Hydroxypentanedioic acid disodium salt
CAS Number 103404-90-6 Molecular Weight 192.078
Density N/A Boiling Point N/A
Molecular Formula C5H6Na2O5 Melting Point >291°C (dec.)
MSDS USA Flash Point N/A

 Use of D-2-Hydroxypentanedioic acid disodium salt


D-alpha-Hydroxyglutaric acid disodium salt is a weak competitive α-Ketoglutarate(α-KG)-dependent dioxygenase inhibitor with Ki of 10.87±1.85 mM. Ki for L-Hydroxyglutaric acid (L-2-HG) is 0.628±0.036 mM.

 Names

Name disodium,(2R)-2-hydroxypentanedioate
Synonym More Synonyms

  Biological Activity

Description D-alpha-Hydroxyglutaric acid disodium salt is a weak competitive α-Ketoglutarate(α-KG)-dependent dioxygenase inhibitor with Ki of 10.87±1.85 mM. Ki for L-Hydroxyglutaric acid (L-2-HG) is 0.628±0.036 mM.
Related Catalog
Target

Ki: 10.87±1.85 mM (α-KG)[1]

In Vitro Addition of 50 mM and 100 mM of D-alpha-Hydroxyglutaric acid (D-2-HG) results in partial and nearly complete inhibition of CeKDM7A, respectively. To further examine the mode of interaction between α-KG and D-2-HG, CeKDM7A are incubated with a fixed concentration (50 mM) of D-2-HG and increasing amount of α-KG. A partial inhibition of KDM7A toward both H3K9me2 and H3K27me2 peptides is observed in the presence of 50 mM D-2-HG and 100 μM α-KG. Addition of 300 μM α-KG is capable of reversing the inhibition of CeKDM7A by 50 mM D-2-HG, indicating that D-2-HG is a weak competitive inhibitor against α-KG toward the CeKDM7A demethylase. D-2-HG with Ki of 10.87±1.85 mM for inhibiting KDM5B/JARID1B/PLU-1[1]. D-alpha-Hydroxyglutaric acid (R-2HG) increases the lifespan of C. elegans. (R)-2HG interacts distinctly with the α-KG dependent dioxygenases. The intracellular (R)-2HG levels are 20-100 fold higher in U87 and HCT 116 cells expressing IDH1(R132H) than in control cells. The elevated (R)-2HG levels are comparable to those found in cells treated with octyl (R)-2HG, and levels reported for IDH1-mutant tumor samples[2].
Kinase Assay To assay human JHDM1A/KDM2A demethylase activity toward H3K36me2, His tagged JHDM1A is first obtained by transforming pET28a-JHDM1A into Escherichia coli BL21 and protein expression is induced by addition of 1 mM IPTG at 30°C when cell density reaches 0.5 OD600 units. Cells are lysed by sonication and Ni-NTA agarose is used to purify His-JHDM1A fusion proteins. Histone demethylase assay is carried out by incubating 2 μg oligonucleosomes, 4 μg purified His-JHDM1A, and/or 10-50 mM D-2-HG in histone demethylation buffer [50 mM HEPES (pH 8.0), 625 μM Fe(NH4)2(SO4)2, 0.1-0.5 mM α-KG, 2 mM ascorbate] at 37°C for 2-3 hr and the reactions are stopped by the addition of SDS loading buffer and subsequently analyzed by western blotting using anti-H3K36me2 antibody. To measure CeKDM7A demethylase activity toward H3K9me2 and H3K27me2, two synthetic dimethylated peptides H3K9me2 [ARTKQTARK (me2)STGGKA] and H3K27me2 [QLATKAARK (me2)SAPAS] are used as substrates. Demethylase assays are carried out in the presence of 10 μg enzyme, 1 μg peptide in 20 μl buffer 20 mM Tris-HCl (pH 7.5), 150 mM NaCl, 50 μM (NH4)2Fe(SO4)2, 100 μM α-KG, 2 mM Vc, 10 mM PMSF for 3 hr. The demethylation reaction mixture is desalted by passing through a C18 ZipTip. To examine the inhibitory effect of 2-HG, various concentrations of 2-HG are incubated with KDM7A briefly before adding other reaction mixtures. The samples are analyzed by a MALDI-TOF/TOF mass spectrometer[1].
Cell Assay U87 cells, HCT 116 IDH1(R132H/+) cells, and HEK 293 cells are seeded in 12-well plates and after overnight incubation are treated with indicated concentrations of each compound (e.g., 400 and 800 μM D-2-HG). After harvesting, cells are stained with Acridine Orange (AO) and DAPI. Cell number and viability are measured based on AO and DAPI fluorescence measured by NC3000[2].
References

[1]. Xu W, et al. Oncometabolite 2-hydroxyglutarate is a competitive inhibitor of α-ketoglutarate-dependent dioxygenases. Cancer Cell. 2011 Jan 18;19(1):17-30.

[2]. Fu X, et al. 2-Hydroxyglutarate Inhibits ATP Synthase and mTOR Signaling. Cell Metab. 2015 Sep 1;22(3):508-15.

 Chemical & Physical Properties

Melting Point >291°C (dec.)
Molecular Formula C5H6Na2O5
Molecular Weight 192.078
Exact Mass 192.001068
PSA 100.49000
Storage condition −20°C

 Safety Information

Personal Protective Equipment Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR NONH for all modes of transport
WGK Germany 3
HS Code 29181990

 Articles1

More Articles
The tumor suppressor prostate apoptosis response-4 (Par-4) is regulated by mutant IDH1 and kills glioma stem cells. Liu Y, Gilbert MR, Kyprianou N, et al.

Acta Neuropathol. 128(5) , 723-32, (2014)

 Synonyms

D-2-Hydroxypentanedioic acid disodium salt
(R)-2-hydroxypentanedioic acid,disodium salt
(R)-2-hydroxy-glutaric acid,disodium-salt
Disodium 2-hydroxypentanedioate
D-(+)-2-hydroxyglutaric acid sodium salt
Pentanedioic acid, 2-hydroxy-, sodium salt (1:2)
D-2-Hydroxyglutaric Acid Disodium Salt
(R)-2-Hydroxy-glutarsaeure,Dinatrium-Salz
D-|A-Hydroxyglutaric acid disodium salt
(2R)-2-Hydroxyglutaric Acid Disodium Salt
Disodium (2R)-2-hydroxypentanedioate
Sodium (R)-2-hydroxypentanedioate
Pentanedioic acid, 2-hydroxy-, sodium salt, (2R)- (1:2)
D-alpha-Hydroxyglutaric acid (disodium salt)
The content on this webpage is sourced from various professional data sources. If you have any questions or concerns regarding the content, please feel free to contact service1@chemsrc.com.
Top Suppliers:I want be here

Get all suppliers and price by the below link:

D-2-Hydroxypentanedioic acid disodium salt suppliers

Price: $66/10mM*1mLinWater

Reference only. check more D-2-Hydroxypentanedioic acid disodium salt price

Related Compounds: More...
1-(2-hydroxyphenyl)pentan-1-one
18430-91-6
D(-)3-PHOSPHOGLYCERIC ACID TRISODIUM SALT
6134-04-9
D-(-)-3-Phosphoglyceric acid disodium
80731-10-8
1,2-Cyclohexanedicarboxylic acid disodium salt
17273-90-4
1,2-Benzenedimethanesulfonic acid disodium salt
97187-28-5
Inosine-5'-diphosphoric acid disodium salt
54735-61-4
L-2-Hydroxyglutaric acid
13095-48-2
(±)-2-Hydroxyglutaric acid
40951-21-1
2-sulfo-octadecanoic acid ; disodium-salt
149635-41-6
2-[Benzyl(methyl)amino]-4-(pyridin-3-yl)pyrimidine-5-carboxylic acid
1775474-51-5
2-Morpholino-4-(3-pyridyl)-5-pyrimidinecarboxylic acid
1775299-18-7
2-[Benzyl(methyl)amino]-4-(pyridin-4-yl)pyrimidine-5-carboxylic acid
1775440-77-1
2-[(2,3-dihydro-1H-inden-1-yl)amino]-4-methylpyrimidine-5-carboxylic acid
1775464-32-8
2-{[2-(1-Cyclohexenyl)ethyl]amino}-4-(methoxymethyl)-5-pyrimidinecarboxylic acid
1775441-50-3
4-(3-Methoxyphenyl)-2-{[(pyridin-3-yl)methyl]amino}pyrimidine-5-carboxylic acid
1775441-66-1
4-(4-Methylphenyl)-2-{[(pyridin-4-yl)methyl]amino}pyrimidine-5-carboxylic acid
1775500-92-9
N-[(1H-indazol-3-yl)methyl]-N-methylhydroxylamine
1517198-56-9
4-(3,5-Difluorophenyl)-2-{[(pyridin-2-yl)methyl]amino}pyrimidine-5-carboxylic acid
1775304-47-6
(2S)-2-amino-2-{imidazo[1,2-a]pyridin-2-yl}ethan-1-ol
2227793-52-2
Chemsrc provides CAS#:103404-90-6 MSDS, density, melting point, boiling point, structure, etc. Its name is D-2-Hydroxypentanedioic acid disodium salt>> amp version: D-2-Hydroxypentanedioic acid disodium salt

Home | MSDS/SDS Database Search | Journals | Product Classification | Biologically Active Compounds | Selling Leads | About Us | Disclaimer

Copyright © 2024 ChemSrc All Rights Reserved