NAI

Modify Date: 2024-01-14 10:24:16

NAI structure	Common Name	NAI
	CAS Number	1055970-47-2	Molecular Weight	187.19800
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₁₀H₉N₃O	Melting Point	N/A
	MSDS	N/A	Flash Point	N/A

Use of NAI

2-Methylnicotinic acid imidazolide, a RNA-SHAPE electrophile, is a general cell-permeable probe of RNA structure. 2-Methylnicotinic acid imidazolide can probe RNA structure in vivo and in live cells with high accuracy and single-nucleotide resolution[1].

Name	3-(1H-imidazol-1-ylcarbonyl)-2-methylpyridine

Description	2-Methylnicotinic acid imidazolide, a RNA-SHAPE electrophile, is a general cell-permeable probe of RNA structure. 2-Methylnicotinic acid imidazolide can probe RNA structure in vivo and in live cells with high accuracy and single-nucleotide resolution[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Application of 100 mM 2-Methylnicotinic acid imidazolide (NAI) to murine embryonic stem cells (ESCs) resulted in 5S rRNA modification in as little as one minute with suitable signal-to-noise ratio. This signal begins to plateau by 15 minutes. 2-Methylnicotinic acid imidazolide also modifies 5S rRNA in cultured human cancer cells, Drosophila S2 cells, yeast, and E. coli. 2-Methylnicotinic acid imidazolide can read out alterations in the RNA tertiary structure as a result of sampling critical mature ribosome conformations.
References	[1]. Robert C Spitale, et al. RNA SHAPE analysis in living cells. Nat Chem Biol. 2013 Jan;9(1):18-20.