| Description |
2-Methylnicotinic acid imidazolide, a RNA-SHAPE electrophile, is a general cell-permeable probe of RNA structure. 2-Methylnicotinic acid imidazolide can probe RNA structure in vivo and in live cells with high accuracy and single-nucleotide resolution[1].
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| Related Catalog |
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| In Vitro |
Application of 100 mM 2-Methylnicotinic acid imidazolide (NAI) to murine embryonic stem cells (ESCs) resulted in 5S rRNA modification in as little as one minute with suitable signal-to-noise ratio. This signal begins to plateau by 15 minutes. 2-Methylnicotinic acid imidazolide also modifies 5S rRNA in cultured human cancer cells, Drosophila S2 cells, yeast, and E. coli. 2-Methylnicotinic acid imidazolide can read out alterations in the RNA tertiary structure as a result of sampling critical mature ribosome conformations.
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| References |
[1]. Robert C Spitale, et al. RNA SHAPE analysis in living cells. Nat Chem Biol. 2013 Jan;9(1):18-20.
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CAS#:58539-65-4
CAS#:1721-23-9