Rhod-2 AM
Modify Date: 2024-01-02 08:37:12
Rhod-2 AM structure
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Common Name | Rhod-2 AM | ||
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| CAS Number | 129787-64-0 | Molecular Weight | 1109.92 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C51H57BrN4O19 | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of Rhod-2 AMRhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm). |
| Name | rhod-2, am |
|---|---|
| Synonym | More Synonyms |
| Description | Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm). |
|---|---|
| Related Catalog | |
| In Vitro | A high concentration of Ca2+ in mitochondria is illustrated by punctate labeling in cells, which is consistent with the location of Ca2+ in the mitochondria following fluorescence staining with Rhod-2 AM (Rhod2-AM) at 6 h p.i.. Inhibition of mitochondrial Ca2+ uptake by ruthenium red (RR) or 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) in IMR5 cells infected with poliovirus (PV) is also illustrated following fluorescence staining with Rhod-2 AM at 6 h p.i.[1]. |
| Cell Assay | For flow cytofluorometry, cells are harvested, pelleted, and resuspended in ice-cold PBS containing 10 mM glucose, 10% fetal bovine serum (FBS), and 10 μM Rhod-2 AM (Rhod2-AM). Mitochondrial calcium levels are determined by the flow cytofluorometry analysis of aliquots of 4×105 cells. For fluorescence microscopy, IMR5 cells are grown on polylysine-coated (10 μg/mL) slides and stained with 7.5 μM Rhod-2 AM in Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS for 2 h before poliovirus (PV) infection. Cells are fixed by incubation for 15 min at 4°C in 4% paraformaldehyde. Cells are washed in PBS, and images are acquired with Zeiss Apotome and Axiovision software[1]. |
| References |
| Molecular Formula | C51H57BrN4O19 |
|---|---|
| Molecular Weight | 1109.92 |
| PSA | 254.73000 |
| LogP | 0.94060 |
| xanthylium |
| 5-tetramethylrhodamine isothiocyanate |
| rhod 2 |
| tetramethylrhodamine-5-isothiocyanate |
| 5-tritc |
| Rhod-2 AM |
- Nanjing Peptide Biotech Ltd
- China
- Product Name: RHOD-2,AM
- Price: ￥Inquiry/10g ￥Inquiry/100g ￥Inquiry/1kg ￥Inquiry/10kg
- Purity: 98.0%
- Stocking Period: 7 Day
- Contact: jom
- Dayang Chem (Hangzhou) Co., Ltd.
- China
- Product Name: RHOD-2, AM
- Price: $Inquiry/100g $Inquiry/1kg $Inquiry/100kg $Inquiry/1000kg
- Purity: 98.0%
- Stocking Period: Inquiry
- Contact: Ms Wang
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Price: $336/1mg
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