Di-8-ANEPPS

Modify Date: 2024-01-02 10:05:47

Di-8-ANEPPS structure	Common Name	Di-8-ANEPPS
	CAS Number	157134-53-7	Molecular Weight	592.87500
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₃₆H₅₂N₂O₃S	Melting Point	N/A
	MSDS	Chinese USA	Flash Point	N/A

Use of Di-8-ANEPPS

Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .

Name	4-(2-[6-(Dioctylamino)-2-naphthalenyl]ethenyl)-1-(3-sulfopropyl)pyridinium inner salt
Synonym	More Synonyms

Description	Di-8-ANEPPS is a naphthylstyryl voltage-sensitive dye, shifting both their fluorescence excitation and emission spectra upon changes in Vm .
Related Catalog	Signaling Pathways >> Others >> Others Dye Reagents Research Areas >> Others
In Vitro	A mouse cell staining with Di-8-ANEPPS is applied voltage clamp pulses and immersed in Na+-containing solution. The Di-8-ANEPPS signal, which largely reflects t system voltage, has an asymmetrical positive component upon application of depolarizing pulses. This is interpreted as reflecting a propagating action potential, escaping from the voltage-clamped plasma membrane. Its temporal properties are not unlike those of the “center” signal. Notably, the peak of the signal occurrs 0.3 ms after the leading edge of the depolarizing pulse[1].
Cell Assay	To implement the technique, cells enzymatically isolated from mouse flexor digitorum brevis (FDB) muscle are stained by brief exposure to a saline with Di-8-ANEPPS and then washed in solution[1].
References	[1]. Manno C, et al. Confocal imaging of transmembrane voltage by SEER of di-8-ANEPPS. J Gen Physiol. 2013 Mar;141(3):371-87.

Molecular Formula	C₃₆H₅₂N₂O₃S
Molecular Weight	592.87500
Exact Mass	592.37000
PSA	72.70000
LogP	9.84120
Storage condition	2-8℃

Di-8-ANEPPS MSDS(Chinese)

Personal Protective Equipment	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
RIDADR	NONH for all modes of transport

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3-[4-[(E)-2-[6-(dioctylamino)naphthalen-2-yl]ethenyl]pyridin-1-ium-1-yl]propane-1-sulfonate

Di-8-ANEPPS