EDTA, AM

Modify Date: 2024-01-17 18:23:55

EDTA, AM Structure
EDTA, AM structure
Common Name EDTA, AM
CAS Number 162303-59-5 Molecular Weight 580.49
Density 1.3±0.1 g/cm3 Boiling Point 607.0±55.0 °C at 760 mmHg
Molecular Formula C22H32N2O16 Melting Point N/A
MSDS N/A Flash Point 320.9±31.5 °C

 Use of EDTA, AM


EDTA-AM (ethylenediaminetetraacetic acid, acetoxymethyl ester) is the membrane-permeant form of the metal chelator EDTA (HY-Y0682). Live cells passively load EDTA-AM by incubating with EDTA-AM. Once internalized, cytoplasmic esterase decomposes AM esters, releasing the active ligand EDTA, which isolates metal ions within the cell. EDTA-AM induces an arrest of mitotic progression and chromosome decondensation[1][2].

 Names

Name EDTA, AM [EDTA, tetra(acetoxyMethyl ester)]
Synonym More Synonyms

 EDTA, AM Biological Activity

Description EDTA-AM (ethylenediaminetetraacetic acid, acetoxymethyl ester) is the membrane-permeant form of the metal chelator EDTA (HY-Y0682). Live cells passively load EDTA-AM by incubating with EDTA-AM. Once internalized, cytoplasmic esterase decomposes AM esters, releasing the active ligand EDTA, which isolates metal ions within the cell. EDTA-AM induces an arrest of mitotic progression and chromosome decondensation[1][2].
Related Catalog
References

[1]. Watkins CS, et.al. Effects on K+ currents in rat cerebellar granule neurones of a membrane-permeable analogue of the calcium chelator BAPTA. Br J Pharmacol. 1996 Aug;118(7):1772-8.  

[2]. Maeshima K, et.al. A Transient Rise in Free Mg2+Ions Released from ATP-Mg Hydrolysis Contributes to Mitotic Chromosome Condensation. Curr Biol. 2018 Feb 5;28(3):444-451.e6.  

 Chemical & Physical Properties

Density 1.3±0.1 g/cm3
Boiling Point 607.0±55.0 °C at 760 mmHg
Molecular Formula C22H32N2O16
Molecular Weight 580.49
Flash Point 320.9±31.5 °C
Exact Mass 580.175171
LogP 2.16
Vapour Pressure 0.0±1.7 mmHg at 25°C
Index of Refraction 1.491

 Synonyms

Acetoxymethyl 8,11-bis[2-(acetoxymethoxy)-2-oxoethyl]-2,6-dioxo-3,5-dioxa-8,11-diazatridecan-13-oate