NBD-PE [N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt]

Modify Date: 2024-01-10 17:15:44

NBD-PE [N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt] structure	Common Name	NBD-PE [N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt]
	CAS Number	178119-00-1	Molecular Weight	956.24000
	Density	N/A	Boiling Point	N/A
	Molecular Formula	C₄₉H₉₀N₅O₁₁P	Melting Point	N/A
	MSDS	N/A	Flash Point	N/A

Use of NBD-PE [N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)-1,2-dihexadecanoyl-sn-glycero-3-phosphoethanolamine, triethylammonium salt]

NBD-PE is an effective lipid fluorescent probe (Excitation/Emission: 465/535 nm; Color: Green). NBD-PE offers a wide array of applications in membrane and cell biology[1][2][3].

Name	nbd-pe

Description	NBD-PE is an effective lipid fluorescent probe (Excitation/Emission: 465/535 nm; Color: Green). NBD-PE offers a wide array of applications in membrane and cell biology[1][2][3].
Related Catalog	Research Areas >> Others Signaling Pathways >> Others >> Others
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Assay of phospholipid transfer activity[3]: 1. The reaction mixture containes 2 mg mitochondrial protein, 0.02 μmol of NBD-PE, 0.5 mmol of Tris-HCl, pH 7.4, and 0-200 μg of pH 5.l-supernatant protein. 2. Samples are incubated at 37°C for 1 hour with gentle agitation. 3. The reaction is terminated by placing samples in an ice bath for 10 min, followed by centrifugation at 15,000 g for 5 min. 4. The supernatants are decanted, allowed to come to room temperature, and the absorbance (460 nm) and the relative fluorescence at 535 nm (excitation at 465 nm) are determined. 5. Blanks containing only NBD-PE and exchange protein are run concomitantly to correct for possible interaction between NBD-PE and exchange protein. 6. The percent exchange of NBD-PE is calculated from the differences between the absorbances or relative fluorescence in the supernatant fractions in the presence and absence of exchange protein.
References	[1]. M Esfahani, et al. 19F-NMR and fluorescence polarization of yeast plasma membrane and isolated lipids. Biochem Biophys Res Commun. 1981 Jul 16;101(1):306-11. [2]. P S Uster, et al. Resonance energy transfer microscopy: visual colocalization of fluorescent lipid probes in liposomes. Methods Enzymol. 1989;171:850-7. [3]. J A Monti, et al. Synthesis and properties of a highly fluorescent derivative of phosphatidylethanolamine. J Lipid Res. 1978 Feb;19(2):222-8.

Molecular Formula	C₄₉H₉₀N₅O₁₁P
Molecular Weight	956.24000
Exact Mass	955.63700
PSA	218.18000
LogP	14.03840
Storage condition	-20°C