LDS 751

Modify Date: 2024-01-20 18:28:24

LDS 751 Structure
LDS 751 structure
Common Name LDS 751
CAS Number 181885-68-7 Molecular Weight 471.976
Density N/A Boiling Point N/A
Molecular Formula C25H30ClN3O4 Melting Point N/A
MSDS N/A Flash Point N/A

 Use of LDS 751


LDS-751 is a nucleic acid stain.

 Names

Name LDS-751
Synonym More Synonyms

 LDS 751 Biological Activity

Description LDS-751 is a nucleic acid stain.
Related Catalog
In Vitro The LDS-751 permits the discrimination of intact cells from residual erythrocyte ghosts, platelets and damaged nucleated cells. The forward and orthogonal fight scattering signals of the intact cells, identified by LDS-751 staining allow a clear separation between the major leukocyte populations since the damaged nucleated cells, erythrocytes, erythrocyte cell ghosts and platelets are removed from the display[1].
Cell Assay Blood samples (1 mL) are obtained using a sterile Butterfly-21 needle and plastic syringe from the antecubital vein of normal healthy volunteers who have given their informed consent. They are immediately transferred to plastic tubes containing 17.3 mg of phenylmethylsulphonyl fluoride (PMSF) and 1 mL of LDS-751 at room temperature. Aliquots (25 μL) are incubated for 5 min with between 2 μL and 5 μL of undiluted monoclonal antibodies, then diluted with 0.5 mL of 1% BSA in Hepesbuffered Hanks' balanced salts solution (HHBSS) and examined by flow cytometry[2].
References

[1]. Terstappen LW, et al. A rapid sample preparation technique for flow cytometric analysis of immunofluorescence allowing absolute enumeration of cell subpopulations. J Immunol Methods. 1989 Sep 29;123(1):103-12.

[2]. McCarthy DA, et al. A simple flow cytometric procedure for the determination of surface antigens on unfixed leucocytes in whole blood. J Immunol Methods. 1993 Aug 9;163(2):155-60.

 Chemical & Physical Properties

Molecular Formula C25H30ClN3O4
Molecular Weight 471.976
Exact Mass 471.192474

 Synonyms

6-(Dimethylamino)-2-{(1E,3E)-4-[4-(dimethylamino)phenyl]-1,3-butadien-1-yl}-1-ethylquinolinium perchlorate