Ac-Leu-Glu-His-Asp-AFC trifluoroacetate salt

Modify Date: 2024-01-11 17:14:44

Ac-Leu-Glu-His-Asp-AFC trifluoroacetate salt structure	Common Name	Ac-Leu-Glu-His-Asp-AFC trifluoroacetate salt
	CAS Number	210345-03-2	Molecular Weight	765.690
	Density	1.5±0.1 g/cm3	Boiling Point	1211.9±65.0 °C at 760 mmHg
	Molecular Formula	C₃₃H₃₈F₃N₇O₁₁	Melting Point	N/A
	MSDS	N/A	Flash Point	686.7±34.3 °C

Use of Ac-Leu-Glu-His-Asp-AFC trifluoroacetate salt

Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase[1].

Name	4-[(2-acetamido-4-methylpentanoyl)amino]-5-[[1-[[3-carboxy-1-oxo-1-[[2-oxo-4-(trifluoromethyl)chromen-7-yl]amino]propan-2-yl]amino]-3-(1H-imidazol-5-yl)-1-oxopropan-2-yl]amino]-5-oxopentanoic acid
Synonym	More Synonyms

Description	Ac-LEHD-AFC is a fluorescent substrate for caspase-9, can be used to assess the activity of caspase[1].
Related Catalog	Research Areas >> Others Signaling Pathways >> Apoptosis >> Caspase
Target	Caspase-9[1]
In Vitro	Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The assessment of the caspase activity by fluorogenic substrate assay[1]: 1. Preparation of cell lysis buffer: 1 % Nonidet P-40, NaCl 200 mM, Tris/HCl 20 mM, pH 7.4, Leupeptin 10 mg/L, Aprotinin (HY-P0017) (0.27 kU/L) and 100 mM PMSF (HY-B0496) . 2. Incubate the lysis buffer (25 mg of total protein) with 50 mmol/L of AC-DEVD-AMC (HY-P1003) (caspase-3), AC-VEID-AFC (caspase-8) or AC-LEHD-AFC (caspase-9) in the buffer (HEPES 10 mM, pH 7.4, containing Mannitol 220 mM, Sucrose 68 mM, NaCl 2 mM, KH2PO4 2.5 mM, EGTA 0.5 mM, MgCl2 2 mM, Pyruvate 5 mM, PMSF 0.1 mM, and Dithiothreitol 1 mM). 3. Assess the caspase activity by measuring fluorescent 7-amino-4-methylcoumarin released for 1 h at a 2-min intervals by a spectrofluorometer.

Density	1.5±0.1 g/cm3
Boiling Point	1211.9±65.0 °C at 760 mmHg
Molecular Formula	C₃₃H₃₈F₃N₇O₁₁
Molecular Weight	765.690
Flash Point	686.7±34.3 °C
Exact Mass	765.258118
PSA	278.99000
LogP	1.45
Vapour Pressure	0.0±0.3 mmHg at 25°C
Index of Refraction	1.587

L-α-Asparagine, N-acetyl-L-leucyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-1-benzopyran-7-yl]-

Acetyl-Leu-Glu-His-Asp-AFC

Ac-LEHD-AFC

AFC154

PASE-9 SUBSTRATE,FLUOROGENIC

N-Acetyl-L-leucyl-L-α-glutamyl-L-histidyl-N-[2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl]-L-α-asparagine

Ac-Leu-Glu-His-Asp-7-Amino-4-trifluoromethylcoumarin

Ac-Leu-Glu-His-Asp-AFC

Shanghai Nianxing Industrial Co., Ltd
China
Product Name: Ac-LEHD-AFC
Price: Click To Check
Purity: 98.0%
Delivery: 10 Day
Contact: Yang
Email: sales@echemcloud.com

Taiwan Oyi peptide Co. Ltd
China
Product Name: Ac-Leu-Glu-His-Asp-AFC trifluoroacetate salt
Price: $Inquiry/1mg $Inquiry/100kg
Purity: 98.0%
Delivery: 1 Day
Contact: Vinnie
Email: Jamie@api-made.com

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