Hoechst 33342 trihydrochloride
Modify Date: 2024-01-05 18:15:40
Hoechst 33342 trihydrochloride structure
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Common Name | Hoechst 33342 trihydrochloride | ||
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| CAS Number | 875756-97-1 | Molecular Weight | 561.93400 | |
| Density | N/A | Boiling Point | N/A | |
| Molecular Formula | C27H31Cl3N6O | Melting Point | N/A | |
| MSDS | N/A | Flash Point | N/A | |
Use of Hoechst 33342 trihydrochlorideHoechst 33342 trihydrochloride is a DNA minor groove binder used fluorochrome for visualizing cellular DNA. |
| Name | 2-(4-ethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole,trihydrochloride |
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| Synonym | More Synonyms |
| Description | Hoechst 33342 trihydrochloride is a DNA minor groove binder used fluorochrome for visualizing cellular DNA. |
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| Related Catalog | |
| Target |
Dye reagent[1] DNA Stain[1] |
| In Vitro | Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. On binding to DNA, the fluorescence greatly increases. This protocol describes the use of Hoechst 33342 to label nuclear DNA of cells grown in culture. Hoechst 33342 can also be used to stain fixed cells by substituting Hoechst 33342 for DAPI[1]. |
| Cell Assay | Labeling Nuclear DNA with Hoechst 33342[1]Step 1, Dilute the Hoechst stock solution 1:100 in H2O for use in labeling. Step 2, Aspirate the cell medium from cells grown on coverslips. Rinse the cells three times with PBS+. Step 3, Incubate the cells in the Hoechst labeling solution (from Step 1) for 10-30 min at room temperature. Step 4, Aspirate the labeling solution. Rinse the cells three times in PBS+.Step 5, Mount the coverslips.Step 6, Image the cells (λex ~353 nm, λem ~483 nm for Hoechst 33342)[1]. |
| References |
| Molecular Formula | C27H31Cl3N6O |
|---|---|
| Molecular Weight | 561.93400 |
| Exact Mass | 560.16200 |
| PSA | 73.07000 |
| LogP | 7.33260 |
| Storage condition | -20℃ |
| Hoechst 33342 (trihydrochloride) |