23-(dipyrrometheneboron difluoride)-24-norcholesterol structure
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Common Name | 23-(dipyrrometheneboron difluoride)-24-norcholesterol | ||
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CAS Number | 878557-19-8 | Molecular Weight | 576.61100 | |
Density | N/A | Boiling Point | N/A | |
Molecular Formula | C36H51BF2N2O | Melting Point | N/A | |
MSDS | N/A | Flash Point | N/A |
Use of 23-(dipyrrometheneboron difluoride)-24-norcholesterolBODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm)[1]. |
Name | Boron, [(3β)-24-(3,5-dimethyl-1H-pyrrol-2-yl-κN)-24-(3,5-dimethyl-2H-pyrrol-2-ylidene-κN)chol-5-en-3-olato]difluoro-, (T-4) |
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Synonym | More Synonyms |
Description | BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm)[1]. |
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Related Catalog | |
In Vitro | BODIPY-cholesterol assay To prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM[1]. Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration[1]. Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 µM), mixed thoroughly and incubated for 10 -20 mins at 37 °C[1]. The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment[1]. |
References |
Molecular Formula | C36H51BF2N2O |
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Molecular Weight | 576.61100 |
Exact Mass | 576.40600 |
PSA | 33.55000 |
LogP | 8.16280 |
Bodipy cholesterol |
TopFluor Cholesterol |
Bodipy FL cholesterol |
23-(dipyrrometheneboron difluoride)-24-norcholesterol |