Combination strategy using pure enzymes and whole cells as biocatalysts for the preparation of 2-hydroxyesters and lactones from 2-oxoglutaric acid

EM Rustoy, EN Pereyra, S Moreno, A Baldessari

Index: Rustoy, Eduardo M.; Pereyra, Elba N.; Moreno, Silvia; Baldessari, Alicia Tetrahedron Asymmetry, 2004 , vol. 15, # 23 p. 3763 - 3768

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Citation Number: 24

Abstract

An innovative combination strategy that uses pure enzymes and whole microbial cells in the same process was used to prepare enantiomerically pure 3-carboxyalkyl-γ-butyrolactones and several alkyl esters of 2-hydroxyglutarates from 2-oxoglutaric acid. The method involves two consecutive biocatalytic steps. The first step, which converts the 2-oxoglutaric acid into the corresponding dialkyl esters, was catalyzed by a lipase. Then in the second step, by ...