Synthesis, incorporation into triplex-forming oligonucleotide, and binding properties of a novel 2'-deoxy-C-nucleoside featuring a 6-(thiazolyl-5) benzimidazole …

D Guianvarc'h, JL Fourrey, R Maurisse, JS Sun…

Index: Guianvarc'h, Dominique; Fourrey, Jean-Louis; Maurisse, Rosalie; Sun, Jian-Sheng; Benhida, Rachid Organic letters, 2002 , vol. 4, # 24 p. 4209 - 4212

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Citation Number: 47

Abstract

6-(Thiazolyl-5) benzimidazole (B t) was designed as a novel nucleobase for the specific recognition of an inverted A⊙ T base pair in a triple helix motif. It was successfully incorporated into an 18-mer triplex-forming oligonucleotide (TFO) using the 2'-deoxy-C- nucleoside phosphoramidite 16. The triple helix binding properties of the modified TFO were examined by means of thermal denaturation experiments targeting an oligopyrimidine⊙ ...