New Monofunctionalized Fluorescein Derivatives for the Efficient High??Throughput Screening of Lipases and Esterases in Aqueous Media

Y Yang, P Babiak, JL Reymond

Index: Yang, Yongzheng; Babiak, Peter; Reymond, Jean-Louis Helvetica Chimica Acta, 2006 , vol. 89, # 3 p. 404 - 415

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Citation Number: 23

Abstract

Abstract Monoalkylation or acylation of fluorescein (1) with various acyloxymethyl or acyl halides afforded, respectively, a series of ether-(2) and ester-functionalized (3) fluorogenic probes. The highly reactive and water-soluble substrates release fluorescein (1) upon reaction with lipases and esterases within seconds or minutes, both under fully aqueous conditions or in the presence of DMSO (20%) as a co-solvent. The most-reactive ...