Tetrahedron

Trityl tags for encoding in combinatorial synthesis

MS Shchepinov, R Chalk, EM Southern

Index: Shchepinov, Mikhail S.; Chalk, Rod; Southern, Edwin M. Tetrahedron, 2000 , vol. 56, # 17 p. 2713 - 2724

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Citation Number: 39

Abstract

New tags and an encoding strategy for combinatorial synthesis are described. Combinatorial libraries of short oligonucleotides attached to TentaGel beads were synthesised by a split-and-mix strategy using 5′-DMTr or 5′-Fmoc-protected nucleoside phosphoramidites. Trityl moieties with different masses were used to tag the nature and position of monomer units (bases) coupled at each step in the synthesis. Beads with a ...