Fast oligonucleotide deprotection phosphoramidite chemistry for DNA synthesis

H Vu, C McCollum, K Jacobson, P Theisen, R Vinayak…

Index: Vu, Huynh; McCollum, Christie; Jacobson, Karen; Theisen, Pete; Vinayak, Ravi; et al. Tetrahedron Letters, 1990 , vol. 31, # 50 p. 7269 - 7272

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Citation Number: 93

Abstract

Abstract A new set of base protecting groups for cyanoethylphosphoramidite nucleosides and supports has been developed which decreases the post-synthesis time requirements. The traditional purine amide protecting groups, which require 8-16 hours at 55 C for deprotection in ammonia, have been replaced with the dimethylformamidine group. Oligonucleotides made with the new reagents require only 1 hour at 55 C or 8 hours at ...