Application of ω-Transaminases in the Pharmaceutical Industry

Stephen A. Kelly, Stefan Pohle, Scott Wharry, Stefan Mix, Christopher C.R. Allen, Thomas S. Moody, Brendan F. Gilmore

Index: 10.1021/acs.chemrev.7b00437

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Abstract

Chiral amines are valuable building blocks for the pharmaceutical industry. ω-TAms have emerged as an exciting option for their synthesis, offering a potential “green alternative” to overcome the drawbacks associated with conventional chemical methods. In this review, we explore the application of ω-TAms for pharmaceutical production. We discuss the diverse array of reactions available involving ω-TAms and process considerations of their use in both kinetic resolution and asymmetric synthesis. With the aid of specific drug intermediates and APIs, we chart the development of ω-TAms using protein engineering and their contribution to elegant one-pot cascades with other enzymes, including carbonyl reductases (CREDs), hydrolases and monoamine oxidases (MAOs), providing a comprehensive overview of their uses, beginning with initial applications through to the present day.