Oligonucleotide synthesis. Part 18. Synthesis of bis (5-chloro-8-quinolyl) nucleoside 5'-phosphates in oligoribonucleotide synthesis by the phosphotriester approach

H Takaku, K Kamaike, K Kasuga

Index: Takaku, Hiroshi; Kamaike, Kazuo; Kasuga, Kenichi Journal of Organic Chemistry, 1982 , vol. 47, # 25 p. 4937 - 4940

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Citation Number: 12

Abstract

A new phosphorylating agent, bis (5-chloro-8-quinolyl) phosphate was found to be a useful agent for the phosphorylation of the 5 '-hydroxyl group of unprotected nucleosides. This agent smoothly reacts with 3', 5'-unprotected nucleosides in the presence of 8- quinolinesulfonyl tetrazolide (QS-t) to give the corresponding bis (5-chloro-8-quinolyl) nucleoside 5'-phosphates in good yields without 3'-mOnO-and 3', 5'-diphosphorylated ...