p-Guanidinobenzoic acid esters of fluorescein as active-site titrants of serine proteases

LL Melhado, SW Peltz, SP Leytus…

Index: Melhado, L. Lee; Peltz, Stuart W.; Leytus, Steven P.; Mangel, Walter F. Journal of the American Chemical Society, 1982 , vol. 104, # 25 p. 7299 - 7306

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Citation Number: 55

Abstract

Abstract: Two active-site titrants of serine proteases, fluorescein mono-p-guanidinobenzoate hydrochloride (FMGBaHCI) and fluorescein di-p-guanidinobenzoate dihydrochloride (FDGB- ZHCI), have been synthesized, purified, and chemically and enzymatically characterized. Electronic absorption and fluorescence emission spectra, fluorescence lifetimes and quantum yields, solubilities, and rates of spontaneous hydrolysis at pH 7-10 are reported. ...