ACS Macro Letters 2018-04-09

B12-Dependent Protein Oligomerization Facilitates Layer-by-Layer Growth of Photo/Thermal Responsive Nanofilms

Jingjing Wei, Wen-Hao Wu, Ri Wang, Zhongguang Yang, Fei Sun, Wen-Bin Zhang

Index: 10.1021/acsmacrolett.8b00147

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Abstract

We report the robust growth of an entirely protein-based, photo- and thermoresponsive Layer-by-Layer nanofilm using genetically encoded SpyTag/SpyCatcher chemistry. The process was facilitated by AdoB12-induced tetramerization of photoreceptor proteins. Protein cargos can be released from the film in a light-dependent manner, showing its potential for therapeutic protein delivery.

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