Genotypic diversity of bacteria and yeasts isolated from Tibetan kefir

Wei Gao, Lanwei Zhang

Index: 10.1111/ijfs.13735

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Abstract

Summary In this study, we have investigated the intraspecies genotypic diversity of bacteria and yeasts isolated from Tibetan kefir via the (GTG)5-REP-PCR technology. According to the cluster analysis of generated fingerprints, forty-nine bacterial isolates were grouped into nine clusters and formed thirteen genotypes, and twenty-two yeast isolates were grouped into two clusters and formed two genotypes. It's worth noting that isolates of Lactobacillus kefiranofaciens, Streptococcus thermophilus, Lactobacillus kefiri and Lactobacillus paracasei exhibited the intraspecies genotypic diversity, whereas isolates of Saccharomyces cerevisiae and Kazachstania unispora did not exhibit. These results confirmed that bacterial isolates possessed higher level of intraspecies genotypic diversity than yeast isolates studied in this research. These findings revealed that (GTG)5-REP-PCR was an efficient and sensitive molecular typing tool for revealing the intraspecies genotypic diversity among the bacterial isolates originated from Tibetan kefir. This study assessed the intraspecies genotypic diversity of bacteria and yeasts isolated from Tibetan kefir via the (GTG)5-REP-PCR technology. The results revealed that (GTG)5-REP-PCR was an efficient and sensitive molecular typing tool for revealing the intraspecies genotypic diversity among the bacterial isolates originated from Tibetan kefir.