Analysis of 10 β-agonists in pork meat using automated dispersive pipette extraction and LC-MS/MS

Kaylee R. Mastrianni, Kalmorat Metavarayuth, William E. Brewer, Qian Wang

Index: 10.1016/j.jchromb.2018.03.026

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Abstract

An analytical procedure for the analysis of 10 β-adrenergic agonists (cimaterol, terbutaline, salbutamol, isoxsuprine, ractopamine, cimbuterol, clenbuterol, brombuterol, mabuterol and mapenterol) in pork meat was developed and validated using LC-MS/MS. An automated dispersive pipette extraction (DPX) was employed on a Hamilton Microlab® NIMBUS96® platform to extract the analytes of interest prior to LC-MS/MS analysis. The extraction time was <20 min with a total LC-MS/MS run time of 9.6 min. The method was fully validated in accordance with the international guidelines (European Commission Decision 2002/657/EC and National Standards of People's Republic of China, GB/T 22286-2008) for limit of detection, limit of quantitation, carryover, extraction efficiency, matrix effects, linearity, and within and between-run precision. The proposed method can be successfully used in the routine determination of 10 β-adrenergic agonists in pork and as a potential solution for compliance monitoring in regulatory laboratories.