Sensors and Actuators B: Chemical 2018-04-04

Ultrasensitive and non-labeling fluorescence assay for biothiols using enhanced silver nanoclusters

Wenmiao Wang, Jian Li, Jialong Fan, Weimin Ning, Bin Liu, Chunyi Tong

Index: 10.1016/j.snb.2018.04.010

Full Text: HTML

Abstract

A label-free, ultra-sensitive and turn-off fluorescence method for detecting cysteine (Cys) has been developed using enhanced DNA-templated silver nanoclusters (DNA-AgNCs) as the fluorescence probe. The method is based on the specific interaction between Cys and DNA-AgNCs via robust Ag-S bonds and the fluorescence quenching ability of Cys to DNA-AgNCs. Using this method for Cys assay, it was found that the change of fluorescent intensity has a good linear relationship with Cys concentration in the range from 0.1 nM to 100 nM (R2 = 0.991). The detection limit of Cys was 0.05 nM. Furthermore, the method was successfully used for the detection of Cys in human serums, the result of which was confirmed using clinical colorimetric assay. In summary, our study shows that this simple, sensitive and rapid detection method can be hopefully used for theranostics.

Latest Articles:

Sensitive and Selective Determination of Caspase-3 Based on Calixarene Functionalized Reduction of Graphene Oxide Assisted Signal Amplification

2018-04-11

[10.1016/j.snb.2018.03.185]

Using statistical methods to carry out in field calibrations of low cost air quality sensors

2018-04-11

[10.1016/j.snb.2018.04.021]

Self-powered smelling electronic-skin based on the piezo-gas-sensor matrix for real-time monitoring the mining environment

2018-04-10

[10.1016/j.snb.2018.04.046]

Naphthalene-Glycine Conjugate: An Extremely Selective Colorimetric Chemosensor for Iodide Ion in Aqueous Solution

2018-04-09

[10.1016/j.snb.2018.04.038]

A Novel Method for the Detection of Silver Ions with Carbon Dots: Excellent Selectivity, Fast Response, Low Detection Limit and Good Applicability

2018-04-09

[10.1016/j.snb.2018.04.036]

More Articles...