Journal of the American Society for Mass Spectrometry 2018-04-06

UV-POSIT: Web-Based Tools for Rapid and Facile Structural Interpretation of Ultraviolet Photodissociation (UVPD) Mass Spectra

Jake Rosenberg, W. Ryan Parker, Michael B. Cammarata, Jennifer S. Brodbelt

Index: 10.1007/s13361-018-1918-x

Full Text: HTML

Abstract

UV-POSIT (Ultraviolet Photodissociation Online Structure Interrogation Tools) is a suite of web-based tools designed to facilitate the rapid interpretation of data from native mass spectrometry experiments making use of 193 nm ultraviolet photodissociation (UVPD). The suite includes four separate utilities which assist in the calculation of fragment ion abundances as a function of backbone cleavage sites and sequence position; the localization of charge sites in intact proteins; the calculation of hydrogen elimination propensity for a-type fragment ions; and mass-offset searching of UVPD spectra to identify unknown modifications and assess false positive fragment identifications. UV-POSIT is implemented as a Python/Flask web application hosted at http://uv-posit.cm.utexas.edu. UV-POSIT is available under the MIT license, and the source code is available at https://github.com/jarosenb/UV_POSIT.

Latest Articles:

Cracking the Sugar Code by Mass Spectrometry

2018-04-11

[10.1007/s13361-018-1912-3]

Distinctive and Complementary MS 2 Fragmentation Characteristics for Identification of Sulfated Sialylated N -Glycopeptides by nanoLC-MS/MS Workflow

2018-04-11

[10.1007/s13361-018-1919-9]

Rapid Quantification of 25-Hydroxyvitamin D 3 in Human Serum by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

2018-04-09

[10.1007/s13361-018-1956-4]

Establishment of a Charge Reversal Derivatization Strategy to Improve the Ionization Efficiency of Limaprost and Investigation of the Fragmentation Patterns of Limaprost Derivatives Via Exclusive Neutral Loss and Survival Yield Method

2018-04-09

[10.1007/s13361-018-1924-z]

Insight into Identification of Acinetobacter Species by Matrix-Assisted Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) in the Clinical Laboratory

2018-04-09

[10.1007/s13361-018-1911-4]

More Articles...