A Fluorescence??Based Assay for Baeyer–Villiger Monooxygenases, Hydroxylases and Lactonases

…, LS Chen, AJ Marsaioli, JL Reymond

Index: Sicard, Renaud; Chen, Lu S.; Marsaioli, Anita J.; Reymond, Jean-Louis Advanced Synthesis and Catalysis, 2005 , vol. 347, # 7-8 p. 1041 - 1050

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Citation Number: 51

Abstract

Abstract Alkylation of umbelliferone and nitrophenol with chloroacetone, 3-chlorobutanone, 2-chlorocyclopentanone and 2-chlorocyclohexanone gave the corresponding 2- coumaryloxy and 2-nitrophenoxy ketones. The 2-coumaryloxy ketones were used as fluorogenic substrates to detect Baeyer–Villiger monooxygenases activities of microbial cultures in high-throughput using microtiter plates. The 2-coumaryloxy ketones were ...